惯性力和介电泳力耦合的细胞分选仿真设计研究

Simulation Design Study on Chip Fabrication of Coupling of Inertial and Dielectrophoresis Forces

针对目前微流控芯片单物理场分选精度低、通量小等劣势,提出了多物理场耦合的分选方式。多物理场粒子分选方案是采用惯性力与介电泳力耦合的方式,通过理论计算分析了惯性力与介电泳力的工作原理,并利用COMSOL对惯性力粒子速度变化、介电泳电场进行了仿真分析,对结构进行了优化,确定了芯片尺寸:整个通道由2部分组成,通道入口处利用惯性力收缩扩张(CEA)完成粒子的聚焦与分离,考虑到分离效率的问题,惯性力进行分选后,加入介电泳进行进一步的分离,以提高芯片的分选精度和分选效率,完成了芯片的加工与制备,对后续粒子的高效分选提供了重要的参考价值。

A multi-physical field coupling separation method was proposed as the disadvantages of low precision and low flux of current microfluidic chips single physical field separation.The coupling mode of inertial force and dieletrophoresis force were adopted in the multi-physical partical sorting scheme.The working principle of inertial force and dielectrophoresis force was analyzed by theoretical calculation,and the velocity change of inertial force particles and dielectrophoresis electric field were simulated and analyzed by COMSOL.The structure was optimized and the chip size was determined.The whole channel was composed of two parts.At the entrance of the channel,the particles were focused and separated by the inertial force contraction and expansion(CEA).In consideration of the separation efficiency,the inertial force was separated and then dielectrophoresis was added for further separation to improve the separation precision and efficiency of the chip,and the chip was processed,which provides an important reference value for the high efficient separation.