骨桥蛋白、环氧化酶-2在宫颈癌组织中的表达及其与化疗敏感性的相关性

Expression of osteopontin and cyclooxygenase-2 in cervical cancer tissues,and their correlation with chemotherapy sensitivity

目的:观察骨桥蛋白(OPN)、环氧合酶-2(COX-2)在宫颈癌组织中的表达,并分析两者与化疗敏感性的相关性。方法:157例行新辅助化疗(NACT)治疗宫颈癌病人,根据相关肿瘤疗效评价标准分为化疗敏感组(112例)和化疗不敏感组(45例)。采用RT-PCR、Western blotting法检测2组病人NACT治疗前后OPN、COX-2 mRNA和蛋白表达水平,分析治疗前宫颈癌组织OPN、COX-2 mRNA表达水平与血管形成指数(VI)、血流指数(FI)、血管血流指数(VFI)等超声相关参数的关系,采用logistic回归法对影响宫颈癌病人化疗敏感性的多因素进行分析。结果:化疗不敏感组治疗前OPN、COX-2 mRNA和蛋白表达水平明显高于化疗敏感组(P<0.01),化疗不敏感组治疗后OPN、COX-2 mRNA和蛋白表达水平未出现明显改变(P>0.05),而化疗敏感组治疗后OPN、COX-2 mRNA和蛋白表达水平明显降低(P<0.01);治疗前宫颈癌组织OPN、COX-2 mRNA表达水平与VI、FI均呈正相关性关系(P<0.05),而与VFI无明显相关性关系(P>0.05);化疗前宫颈癌组织OPN、COX-2 mRNA表达水平及VI、FI为影响化疗敏感性的独立危险因素(P<0.05)。结论:宫颈癌组织中OPN、COX-2 mRNA和蛋白均呈高表达状态,且两者与化疗敏感性之间存在密切的联系。

Objective:To observe the expression levels of osteopontin(OPN)and cyclooxygenase-2(COX-2)in cervical cancer tissues,and analyze their correlation with the chemotherapy sensitivity.Methods:One hundred and fifty-seven cervical cancer patients treated with neoadjuvant chemotherapy(NACT)were divided into the sensitive chemotherapy group(112 cases)and insensitive chemotherapy group(45 cases)according to the relevant tumor efficacy evaluation criteria.The mRNA and protein levels of OPN and COX-2 in two groups before and after NACT treatment were detected using RT-PCR and Western blotting,respectively.The relationship between the expression levels of OPN and COX-2 mRNA and ultrasonic parameters[blood vascularization index(VI),flow index(FI)and vascularization flow index(VFI)]in cervical cancer before treatment were analyzed.The multiple factors influencing the chemotherapy sensitivity in patients with cervical cancer were analyzed using logistic regression analysis.Results:Before treatment,the expression levels of OPN and COX-2 mRNA and protein in insensitive chemotherapy group were significantly higher than those in sensitive chemotherapy group(P<0.01).After chemotherapy,there were not significant changes in the expression levels of OPN and COX-2 mRNA and protein in insensitive chemotherapy group(P>0.05),and the expression levels of OPN,COX-2 mRNA and protein in the sensitive chemotherapy group significantly reduced(P<0.01).Before treatment,the expression levels of OPN and COX-2 mRNA in cervical cancer tissues were positively correlated with VI and FI(P<0.05),and not significantly correlated with VFI(P>0.05).The expression levels of OPN and COX-2 mRNA,VI and FI in cervical cancer tissues before chemotherapy were the independent risk factors for chemotherapy sensitivity(P<0.05).Conclusions:The expression levels of OPN,COX-2 mRNA and protein in cervical cancer tissues are high,and both are closely related to the chemotherapy sensitivity.