高效液相色谱法测定水果和蔬菜中维生素C含量的不确定度评定

Uncertainty evaluation of the determination of vitamin C in fruits and vegetables by high performance liquid chromatography

通过分析维生素C测定过程中不确定度的来源,建立数学模型,评定出各不确定度分量,计算出合成标准不确定度和扩展不确定度。结果表明,当样品中维生素C含量为33.7 mg/100 g时,扩展不确定度为1.4 mg/100 g(k=2);标准储备溶液的配制和测量重复性是不确定度的主要来源。

To evaluate the uncertainty of the determination of vitamin C in fruits and vegetables by high performance liquid chromatography,a mathematical model was established to evaluate each uncertainty component,and the standard uncertainty and extended uncertainty of the synthesis were calculated.The results showed that the amounts of vitamin C in samples was 33.7 mg/100 g,and the expanded uncertainty was 1.4 mg/100 g(k=2).The uncertainties of the determination mainly come from the preparation of standard solution and the repeatability of determination.