朝鲜蓟茎叶中脂溶性提取物对神经细胞的保护作用

Protective Effect of Lipophilic Extracts from the Stems and Leaves of Global Artichoke on Nerve Cells

目的:探究朝鲜蓟茎叶中脂溶性提取物萜类及甾醇类化合物的组成与含量,探究脂溶性提取物对H_(2)O_(2)诱导的PC12神经细胞的保护作用。方法:采用GC-MS分析朝鲜蓟副产物中萜类及甾醇类化合物的组成与含量,以脂溶性提取物为保护剂,对经H_(2)O_(2)诱导伤害的PC12细胞进行预处理,检测细胞存活率,乳酸脱氢酶(LDH)漏出率、丙二醛(MDA)含量,活性氧(ROS)水平及细胞凋亡率。结果:从朝鲜蓟茎叶副产物中共检测到3种倍半萜内脂,2种甾醇及8种五环三萜,其中菜蓟苦素含量最高,达13309.10 mg/kg DM。经朝鲜蓟副产物脂溶性提取物保护后,PC12神经细胞存活率显著升高,细胞内LDH漏出率、MDA含量及ROS水平显著降低。经Annexin V-FITC/PI双染及AO-EB双染后,细胞凋亡率呈显著降低趋势。结论:朝鲜蓟副产物脂溶性提取物对PC12神经细胞具有保护作用。

Objective:To investigate the composition and content of terpenoids and sterols in the lipophilic extract of artichoke by-products,and to explore the protective effect of lipophilic extract on PC12 nerve cells induced by H_(2)O_(2).Methods:Composition and content of terpenoids and sterols in artichoke byproducts were analyzed by GC-MS.Liposoluble extracts were used as protective drugs to protect PC12 cells injured by H_(2)O_(2).Cell viability,LDH release,MDA production,ROS level and apoptosis rate were detected.Results:Three sesquiterpene lactones,two sterols and eight pentacyclic triterpenoids were detected in the by-products of artichoke.The content of cynaropicrin was the highest,up to 13309.10 mg/kg DM.After being protected by lipophilic extract of artichoke byproducts,the cell viability of PC12 nerve cells increased significantly,the release rate of LDH,MDA content and ROS level in cells decreased significantly.After double staining by Annexin V-FITC/PI and AO-EB,the apoptosis rate of PC12 cells also showed a significant decreasing trend.Conclusion:Lipophilic extract of artichoke by-product has protective effect on PC12 nerve cells.