荞麦蜂花粉多糖对人体粪便菌群代谢的影响

Effects of Polysaccharide from Fagopyrum esculentum Moench Bee Pollen on Metabolism of Human Fecal Flora

本文主要研究荞麦蜂花粉多糖(WFPP)在体外发酵过程中对人体粪便菌群代谢产物的影响。通过建立体外发酵模型,测定发酵液中pH值、总糖含量、NH_(3)-N含量及短链脂肪酸含量,分析WFPP体外酵解规律,探究WFPP对人体肠道菌群的有益作用。随着发酵时间的延长,发酵液中pH值和总糖含量不断降低,发酵24 h后,WFPP-H组pH值由7.26降至4.64,多糖组总糖消耗量均在80%以上。添加WFPP能明显减少培养液中NH_(3)-N的积累,且添加量越高效果越明显。乙酸、丙酸、正丁酸是发酵液中主要的短链脂肪酸(SCFAs),在发酵过程中其含量都显著增加,各组发酵产物中短链脂肪酸含量高低排序为WFPP-H>WFPP-M>WFPP-L。结论:荞麦蜂花粉多糖能被人体肠道菌群酵解,在产生短链脂肪酸的同时降低培养液中pH值,减少NH_(3)-N的积累,并具有一定的剂量关系。荞麦蜂花粉多糖具有潜在的益生元作用。

The study investigated the in vitro fermentation of polysaccharide from Fagopyrum esculentum moench bee pollen(WFPP),the effects of WFPP on the metabolites produced by human intestinal flora.By establishing an in vitro fermentation model,the pH value,total sugar content,NH_(3)-N content and SCFAs content of fermentation broth were determined.The fermentation behavior of WFPP was analyzed to explore the beneficial effects of WFPP on human intestinal flora.The pH value and content of total sugar in the fecal culture declined as fermentation time increased,after 24 h of fermentation,the pH value of WFPP-H group decreased from 7.26 to 4.64,the percentage of the total carbohydrates consumed in WFPP groups reached up to 80%.The accumulation of NH_(3)-N content was reduced significantly by adding WFPP in culture broth,and the higher the content of WFPP,the better the effect.Acetic,propionic and N-butyric acid were the dominant short-chain fatty acids(SCFAs),which got a significantly rise in fermentation,the content of SCFAs in the culture broth of polysaccharide groups was WFPP-H>WFPP-M>WFPP-L.The results showed that WFPP could be hydrolyzed be human intestinal flora,which produced SCFAs lowered the pH value,reduced the accumulation of NH_(3)-N,the WFPP have a potential prebiotic effect.