基于网络药理学的四妙勇安汤治疗深静脉血栓形成机制研究

Study on mechanism of Simiao Yong’an Decoction in treatment of deep venous thrombosis based on network pharmacology

目的:通过网络药理学方法对四妙勇安汤治疗深静脉血栓形成的作用机制进行探索。方法:通过TCMSP数据库获取四妙勇安汤中各味中药的有效成分并预测各有效成分的作用靶点;通过CTD数据库获取深静脉血栓形成的靶点,并将成分作用靶点与疾病靶点取交集得到关键靶点;通过String数据库构建蛋白-蛋白相互作用(PPI)网络;运用Cytoscape 3.7.2软件构建药物-成分-靶点-疾病网络图;Clue GO插件进行四妙勇安汤治疗深静脉血栓形成GO功能和KEGG通路富集分析。结果:获得四妙勇安汤121个有效成分,137个潜在靶点,深静脉血栓形成1172个靶点,GO Biological Process分析得到214条生物过程,KEGG分析得到72条通路。结论:四妙勇安汤可能通过作用于ESR1、AR、PTGS2等关键靶点以及AGE-RAGE信号通路、PI3K-Akt信号通路、MAPK信号通路、IL-17信号通路、TNF信号通路、RLX信号通路等达到治疗深静脉血栓形成的作用。

Objective:To explore the mechanism of Simiao Yong’an Decoction in treating deep venous thrombosis based on the method of network pharmacology.Methods:Through TCMSP database,the effective components of each traditional Chinese medicine in Simiao Yong’an Decoction were obtained and their targets were predicted.The targets of deep venous thrombosis were collected by CTD database,and the key targets were obtained by intersection of the component targets and the disease targets;protein-protein interaction(PPI)network was constructed by String database.The drugs-components-targets-disease network map was constructed by using the software of Cytoscape 3.7.2.GO function and KEGG pathway enrichment of Simiao Yong’an Decoction in the treatment of deep venous thrombosis were analyzed by using the plug-in of ClueGo.Results:121 effective components and 137 potential targets of Simiao Yong’an Decoction were obtained.There were 1172 targets of deep venous thrombosis.There were 214 biological processes analyzed by GO biological process and 72 pathways analyzed by KEGG analysis.Conclusion:Simiao Yong’an Decoction may act on ESR1,AR,PTGS2 and other key targets,as well as AGE-RAGE signaling pathway,PI3 K-Akt signaling pathway,MAPK signaling pathway,IL-17 signaling pathway,TNF signaling pathway,RLX signaling pathway and other pathways to treat deep venous thrombosis.