摘要
为观察含人参茎叶皂苷(GSLS)和硒(Se)的溶液(GSe)对猪伪狂犬病(PR)疫苗免疫的影响,本研究给小鼠免疫PR疫苗前注射含GSLS和Se的灭菌溶液,测定免疫后小鼠血清中特异性gB抗体及其亚类水平,淋巴细胞增殖能力,淋巴细胞分泌细胞因子(IFN-γ、IL-2、IL-4、IL-6和IL-10)水平以及猪伪狂犬病病毒(PRV)攻毒后小鼠的存活率,并观察GSe对巨噬细胞RAW 264.7的影响。结果表明,与对照组比较,在PR疫苗免疫前注射GSe可显著提高gB抗体及其亚类IgG1和IgG2a水平、淋巴细胞增殖水平、细胞因子(IFN-γ、IL-2、IL-4、IL-6和IL-10)和小鼠对PRV攻毒的抵抗力。同时,GSe提高了巨噬细胞分泌IL-6、IL-10和TNF-α水平以及细胞表面共刺激分子CD40、CD80和CD86的表达水平。因此,GSe可通过增强动物的固有免疫提高对PR疫苗的免疫应答。
To determine the immunoenhancement effect of GSe solution containing ginseng stemleaf saponins(GSLS)and selenium(Se)on the pseudorabies(PR)vaccine,mice were injected with sterile GSe solution containing GSLS and Se at pre-immunization of PR vaccine(GSe/pre-PRV).Results showed GSe/pre-PRV induced significantly higher serum gB-specific antibody responses(IgG,IgG1and IgG2a),increased lymphocyte proliferation,induced higher production of cytokines(IFN-γ,IL-2,IL-4,IL-6and IL-10)by splenocytes,improved the survival rate of immunized mice challenged with PRV.Additionally,GSe could improve the IL-6,IL-10and TNF-αproductions as well as the costimulatory molecules CD40,CD80and CD86expression of macrophages.In conclusion,GSe can enhance the immune responses of mice to the PRV vaccine by improving the innate immune responses.
作者
王勇
袁丽佳
胡松华
WANG Yong;YUAN Lijia;HU Songhua(College of Animal Sciences,Zhejiang University,Hangzhou 310058,China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2021年第4期735-740,共6页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(31672598)。
