摘要
MicroRNAs(miRNAs)are one of the most important regulators of gene expression.Their biogenesis starts with the transcription of primary miRNA transcripts(pri-miRNAs).In plants,the RNase Ⅲ enzyme DICER-LIKER 1(DCL1),together with its partner,the double-stranded RNA-binding protein HYPONASTIC LEAVES 1(HYL1),and the zinc-finger protein SERRATE(SE),process the imperfect miRNA-residing stem loop of pri-miRNAs to release the miRNA/miRNA*duplexes in the nucleus,which are then methylated by HUA1 ENHANCER 1(Rogers and Chen,2013;Song et al.,2019).Then,most miRNAs are sorted into AGONAUTE 1 to mediate mRNA-cleavage or translation inhibi-tion based on sequence complementarity(Rogers and Chen,2013;Song et al.,2019).Studies have shown that miRNA biogenesis is controlled through modulating pri-miRNA transcription,stability and processing,which is often coupled with various developmental and environmental signals.
基金
This work is supported by grants from NIH(GM127414 to B.Y.)
NSF(MCB-1818082 to B.Y.).
