摘要
N6-methyladenosine(m^(6)A),a ubiquitous internal modification of eukaryotic mRNAs,plays a vital role in almost every aspect of mRNA metabolism.However,there is little evidence documenting the role of m^(6)A in regulating alternative polyadenylation(APA)in plants.APA is controlled by a large protein-RNA complex with many components,including CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR30(CPSF30).In Arabidopsis,CPSF30 has two isoforms and the longer isoform(CPSF30-L)contains a YT512-B Homology(YTH)domain,which is unique to plants.In this study,we showed that CPSF30-L YTH domain binds to m^(6)A in v itro.In the cpsf30-2 mutant,the transcripts of many genes including several important nitrate signaling-related genes had shifts in polyadenylation sites that were correlated with m^(6)A peaks,indicating that these gene transcripts carrying m^(6)A tend to be regulated by APA.Wild-type CPSF30-L could rescue the defects in APA and nitrate metabolism in cpsf30-2,but m^(6)A-binding-defective mutants of CPSF30-L could not.Taken together,our results demonstrated that m^(6)A modification regulates APA in Arabidops is and revealed that the m^(6)A reader CPSF30-L affects nitrate signaling by controlling APA,shedding new light on the roles of the m^(6)A modification during RNA 3-end processing in nitrate metabolism.
基金
This work was supported by grants from the National Natural Science Foundation of China(31788103 to X.C.,31670247 to Y.W.,31870755 to S.L.,31801063 to Y.H.,31701096 to J.S.,31900435 to B.W.)
the Chinese Academy of Sciences(Strategic Priority Research Program XDB27030201 and QYZDY-SSW-SMC022 to X.C.)
the Guangdong Innovation Research Team Fund(2016ZT06S172 to S.L.)
the Shenzhen Sci-Tech Fund(No.KYTDPT20181011104005 to S.L)
the China Postdoctoral Science Foundation(2016M600143 to Y.H.)
the Guangdong Science and Technology Department(2020B1212060018 and 2020B1212030004 to B.W.).
