URG4基因促进肝癌细胞迁移和侵袭

URG4 gene promotes migration and invasion of hepatocellular carcinoma cells

目的探讨上调基因4(URG4)对人肝癌细胞系Hep3B细胞迁移、侵袭的作用。方法通过定量PCR检测转染干扰RNA(siRNA)后Hep3B细胞内URG4 mRNA变化,通过Western blotting检测转染siRNA后Hep3B细胞内URG4蛋白变化。分别对转染后的细胞采取划痕实验、Transwell实验检测URG4基因对Hep3B细胞迁移以及侵袭能力的影响。最后通过Western blotting方法检测URG4敲降对Hep3B细胞内TGF-β和E-cadherin表达的影响。结果siURG4不仅可显著降低Hep3B细胞内URG4 mRNA水平(P<0.05),同时还可以显著降低Hep3B细胞内URG4蛋白水平(P<0.05)。URG4敲降引起Hep3B细胞迁移和侵袭能力明显减弱(P<0.05)。除此之外,URG4敲降引起Hep3B细胞内TGF-β蛋白水平降低和E-cadherin蛋白水平上升。结论URG4通过调控TGF-β和E-cadherin基因的表达,从而降低Hep3B细胞的迁移、侵袭能力。这为继续展开对于肝癌的发生、发展过程的研究提供了新的思路。

Objective To investigate the role of upregulation of cell proliferation gene(URG4)in the migration and invasion of human hepatoma cell line Hep3B cells.MethodsThe changes of URG4 expression in Hep3B cells after transfection of siRNA were detected by quantitative PCR and western blotting.The wound healing and the Transwell assay were used to detect the effect of URG4 knockdown on the migration and invasion of Hep3B cells.And the effect of URG4 knockdown on the expression of TGF-βand E-cadherin in Hep3B cells were detected by western blotting.ResultssiURG4 did not only significantly reduced the level of URG4 mRNA(P<0.05),but also significantly reduced the level of URG4 protein in Hep3B cells(P<0.05).Knockdown of URG4 caused a significant decrease of migration and invasion in Hep3B cell(P<0.05).In addition,URG4 knockdown caused a decrease in TGF-βprotein level but an increase in E-cadherin protein level in Hep3B cells.ConclusionURG4 could participate in the migration and invasion of Hep3B cells by regulating TGF-βand E-cadherin,which provides new ideas for in-depth understanding of the occurrence and development of hepatocellular carcinoma.