YAP蛋白在结直肠癌组织中的表达情况及临床意义的研究

Expression of YAP protein in colorectal cancer tissues and its clinical significance

目的研究YAP蛋白在结直肠癌组织中的表达情况及其与临床病理特点的关系,探讨其在预后评估中的价值。方法收集结直肠癌、癌旁及正常肠黏膜石蜡标本各80例,使用免疫组化法检测YAP蛋白的表达,分析不同性别、年龄、TNM分期、分化程度分组中YAP蛋白的阳性表达率。采用Kaplan-Meier法绘制YAP蛋白不同表达组的结直肠癌患者的生存函数曲线并比较其预后。结果YAP蛋白在结直肠癌、癌旁以及正常肠黏膜组中的阳性表达率分别为67.50%,27.50%和23.75%。不同性别、年龄、肿瘤直径组YAP蛋白的表达阳性率无统计学差异(P>0.05)。不同TNM分期、分化程度组中YAP蛋白的表达阳性率有差异(P<0.05)。入组结直肠癌患者平均生存期为29.638,标准误为1.228,生存函数曲线中YAP阴性组患者生存率高于YAP阳性组(P<0.05)。结论YAP蛋白在结直肠癌患者组织中呈高表达,其表达与结直肠癌临床病理特点及预后有关,有望成为结直肠癌诊断及预后评估的一项参考指标。

ObjectiveTo investigate the expression of YAP protein in colorectal cancer tissues,and the relationship between YAP protein and clinicopathological features.MethodsParaffin specimens were collected from 80 cases of colorectal cancer tissues.The expression of YAP protein was detected by immunohistochemistry.And the positive expression rate of Yap protein in colorectal cancer tissues with different gender,age,TNM stage and differentiation degree was analyzed.Kaplan-Meier method was used to draw the survival function curve of colorectal cancer patients,and to compare the survival rate of colorectal cancer patients with different YAP protein expression.ResultsThe positive expression rates of YAP protein in colorectal cancer,adjacent tissues and normal intestinal mucosa were respectively 67.5%,27.5%and 23.75%.There was no significant difference in the expression of YAP protein in different gender,age and tumor diameter groups(P>0.05).The positive rate of YAP protein expression in colorectal cancer tissues was significantly different with different tumor stages and differentiation degrees(P<0.05).The average survival time of colorectal cancer patients was 29.638,and the standard error was 1.228.Kaplan-Meier method was used to draw the survival function curve of patients with colorectal cancer in YAP negative group and positive group.The curve indicated that the survival rate of patients in YAP negative group was higher than that in YAP positive group(P<0.05).ConclusionYAP protein is highly expressed in the tissues of patients with colorectal cancer,and its expression is related to the clinicopathological characteristics of colorectal cancer.YAP protein is expected to be an reference indicator for diagnosis and prognosis assessment of colorectal cancer patients.