响应面法优化棕色棉纤维原花青素的提取工艺

Proanthocyanidin Extraction Process from Brown Cotton Fiber:Optimized by Response Surface Method

为了提高棕色棉纤维中原花青素的得率,优化原花青素的提取工艺。本文以棕色棉纤维为材料,研究甲醇浓度、料液比、超声时间和超声温度等单因素对原花青素提取率的影响,获得优化的提取条件。通过超声与甲醇-盐酸正丁醇相结合的方法提取原花青素,响应面法优化棕色棉纤维原花青素的提取工艺为:甲醇浓度83%,料液比1:60,超声温度42℃,超声时间18 min,提取率为(7.16±0.03)%,与一般方法相比,原花青素提取率提高4%左右。响应面法优化的提取工艺提取效率高,简单快速且可进行批量提取,为原花青素的生物活性研究和产品开发奠定基础。

To improve the yield of proanthocyanidin from brown cotton fiber, and optimize the extraction process, the brown cotton fibers were used as materials to study the effect of methanol concentration, solid-liquid ratio, ultrasonic time and temperature on the extraction efficiency of proanthocyanidin, and to obtain the optimized extraction conditions. Proanthocyanidin was extracted by ultrasonic combined with methanol-n-butanol hydrochloride, the extraction process was optimized by response surface methodology. The results showed that the optimum parameters were methanol concentration of 83%, solid-liquid ratio of 1:60, ultrasonic temperature of 42℃, ultrasonic time of 18 min, and the extraction rate was(7.16±0.03)%. The extraction yield of proanthocyanidin by the optimized method was improved by about 4% compared with that of the general method. The extraction process optimized by response surface methodology has high extraction efficiency, and the experimental operation is simple and fast, which can be used to extract proanthocyanidin in batches. The study lays a foundation for the research on biological activities and the development of products of proanthocyanidin.