聚苹果酸高产菌的筛选与鉴定

Screening and Identification of Poly Malic Acid Producing Strains

从校园周边取样进行了聚苹果酸(Poly malic acid, PMA)高产菌株的分离筛选。经离心管发酵初筛获得了21株产PMA菌株,并初步判断编号Ⅰ-2、Ⅰ-13a、Ⅲ-16菌株的产PMA能力较强,经摇瓶发酵复筛,确定编号Ⅰ-13a菌株的产PMA能力最强,产量达到(43.08±0.36) g/L。通过形态学特征观察和分子生物学分析,对高产菌株进行了菌种鉴定,结果表明该菌株生长初期呈浅粉色,后期变绿变暗;细胞呈芽殖,卵圆形,单个存在;ITS核苷酸序列长度为560 bp,与Aureobasidium melanogenum菌株同源相似度为97%。由此确定该菌株为产黑色素短梗霉(Aureobasidium melanogenum),命名为ZUST-HD,GenBank库登录号为MK754072.1。

Poly malic acid(PMA)producing strains were isolated and screened from the campus.Twenty-one PMA producing strains were obtained by centrifugal tube primary screening,and it was preliminarily judged that the strains I-2,Ⅰ-13 a andⅢ-16 had strong PMA producing capacity.It was determined that strainⅠ-13 a had the strongest PMA production capacity,and the yield reached(43.08±0.36)g/L after shake flask secondary screening.The high yield strains were identified by morphological observation and molecular biological identification.The results showed that the strain was light pink at the initial stage of growth,and turned green and dark at the later stage.The cells were budding,oval and single.The length of ITS nucleotide sequence was 560 bp,and the homology similarity with Aureobasidium melanogenum was 97%.Finally,the strain was identified as Aureobasidium melanogenum and named as ZUST-HD.The GenBank library accession number of it was MK754072.1.