黑、白藜麦皂苷的提取及其抗氧化活性的比较

Extraction of Saponins from Black and White Quinoa and Comparison of Their Antioxidant Activities

本研究以黑、白藜麦种子为试验材料,采用甲醇回流提取法,提取黑、白藜麦种子中的总皂苷;以总皂苷提取率为重要指标,对比黑、白藜麦种子皂苷含量之间的差异;同时,通过测定黑、白藜麦种子皂苷的还原(Fe^(3+))能力和清除羟自由基(·OH)、亚硝酸根离子(NO_(2)^(-))、超氧阴离子(O_(2)^(-)·)及1, 1-二苯基-2-苦基肼自由基(DPPH·)的能力,分析黑、白藜麦种子皂苷的抗氧化活性。结果表明:黑藜麦种子皂苷含量高于白藜麦种子皂苷含量,分别为17.37和1.89 mg/g。此外,藜麦的还原力和抗氧化活性随皂苷质量浓度的增大而增强;黑藜麦种子皂苷的还原力和抗氧化活性高于白藜麦种子皂苷;黑藜麦种子皂苷对DPPH·、O_(2)^(-)·、·OH、NO_(2)^(-)的IC_(50)值分别为0.122、1.106、0.514、0.832 mg/mL;白藜麦种子皂苷对DPPH·、O_(2)^(-)·、·OH、NO_(2)^(-)的IC_(50)值分别为0.210、5.530、2.500、1.569 mg/mL。

Black and white quinoa seeds were used as materials in this study,and the methanol reflux extraction method was used to extract the total saponins from the quinoa seeds.The total saponins extraction rate was an important indicator,and the saponins content was compared the difference between the black and white quinoa seeds.At the same time,the reduction(Fe^(3+))ability,the hydroxyl radical(·OH),nitrite ion(NO_(2)^(-)),superoxide anion(O_(2)^(-)·)and 1,1-Diphenyl-2-picrylhydrazine(DPPH·)scavenging ability were determined to analyze the antioxidant activity of black and white quinoa saponins.The results showed that the saponins content of black quinoa seeds was higher than that of white quinoa seeds,which were 17.37 and 1.89 mg/g,respectively.In addition,the reducing power and antioxidant activity of quinoa increased with the increase of concentration of saponins.Moreover,the reducing power and antioxidant activity of black quinoa seed saponins were higher than those of white quinoa seed saponins.As a contrast,the IC_(50) values of black quinoa seed saponins for DPPH·,O_(2)^(-)·,·OH,NO_(2)^(-)were 0.122,1.106,0.514,0.832 mg/mL,respectively.The IC_(50) values of white quinoa seed saponins for DPPH·,O_(2)^(-)·,·OH,NO_(2)^(-)were 0.210,5.530,2.500,1.569 mg/mL,respectively.