广陈皮中多甲氧基黄酮提取物对脂多糖诱导小鼠急性肺损伤的保护作用

Protective effects of polymethoxyflavones extract from Citrus reticulata‘Chachi’on acute lung injury induced by lipopolysaccharide in mice

为研究广陈皮中多甲氧基黄酮提取物对脂多糖诱导小鼠急性肺损伤的保护作用,先对广陈皮中多甲氧基黄酮进行提取、分离、纯化和结构鉴定,再采用鼻腔滴注脂多糖法建立小鼠急性肺损伤模型,通过测定小鼠肺组织损伤程度、肺组织湿/干质量比、氧化损伤指标活性和促炎细胞因子含量等,探究其对急性肺损伤的影响。结果显示,广陈皮中多甲氧基黄酮提取物的主要成分为川陈皮素和桔皮素(约占多甲氧基黄酮提取物的80%),纯化后川陈皮素纯度可达95%;250 mg/kg多甲氧基黄酮提取物和50 mg/kg川陈皮素能显著缓解小鼠急性肺损伤。研究结果表明,多甲氧基黄酮提取物和川陈皮素具有良好的抗氧化和抗炎效果,对急性肺损伤具有一定的保护作用。

Polymethoxyflavones(PMFs)in citrus have been of increasing interest due to its extensive biological activities.Nobiletin(NOB),one of the main substances of PMFs,has been reported to exhibit anti-oxidant,anti-inflammatory,anti-cancer,and nerve protection resistance activities.Although the anti-inflammatory activity of PMFs has been already reported,its involvement in lung protection has rarely been reported.This article is aimed to investigate the protective effects of PMFs in Citrus reticulata‘Chachi’on acute lung injury(ALI)induced by lipopolysaccharide in mice.PMFs were extracted from Citrus reticulata‘Chachi’and purified.The structure of PMFs in citrus was identified.A mouse model of acute lung injury was established by intranasal instillation of lipopolysaccharide.The effects of PMFs on ALI were explored by measuring the degree of lung tissue damage,the wet/dry mass ratio of lung tissue,the activity of oxidative damage indicators,and the content of pro-inflammatory cytokines.The results showed that the main components of the extracted PMFs were the nobiletin and tangeretin(About 80%in the PMFs).PMFs with the contents of 250 mg/kg and NOB with the contents of 50 mg/kg significantly alleviated ALI.It is indicated that PMFs and NOB have good anti-oxidant and anti-inflammatory effects,and have a certain protective effect on ALI.