盐酸-壳聚糖絮凝耦合处理回收鲢鱼糜漂洗液中的肌浆蛋白

Hydrochloric acid-chitosan flocculation coupling treatment for recovery of sarcoplasmic protein from surimi rinsing solution of silver carp

为提高鱼糜漂洗液中肌浆蛋白的利用率,以鲢鱼糜为研究对象,采用单一壳聚糖絮凝法和盐酸-壳聚糖絮凝耦合处理回收鲢鱼糜漂洗液中的肌浆蛋白,并通过单因素试验和Box-Bechnken中心组合设计对盐酸-壳聚糖絮凝耦合处理的絮凝条件进行优化。结果发现:盐酸处理(pH 3.0)可以显著提高壳聚糖作为单一絮凝剂时的肌浆蛋白回收率(从35.52%提高到81.54%),肌浆蛋白的最佳回收工艺确定为:pH 6.5,壳聚糖用量250 mg/L,温度30℃,时间90 min。在此条件下,可以获得85.23%的高蛋白质回收率以及73.32%的COD去除率。同时,此方法引入鱼糜漂洗液中的NaCl质量分数为0.006%,不会使后续废水处理成本增加,具有一定的可行性。

The sarcoplasmic protein is a water-soluble protein,which accounts for 20%-40%of fish protein.It is lost with the rinsing liquid during the preparation of surimi,resulting in a waste of protein resources.In order to improve the utilization rate of sarcoplasmic protein in surimi rinsing solution,the single chitosan flocculation and hydrochloric acid-chitosan flocculation coupling treatment were used to recover the sarcoplasm protein from surimi rinsing solution of silver carp.The flocculation conditions of hydrochloric acid-chitosan flocculation coupling treatment were optimized through single factor experiments and Box-Bechnken central combination design.The results showed that the recovery rate of sarcoplasmic protein was significantly increased from 35.52%to 81.54%by hydrochloric acid treatment(pH 3.0)when chitosan was used as a single flocculant.The recovery process of sarcoplasmic protein was optimized as follows:pH 6.5,250 mg/L chitosan,30℃,90 min.Under these conditions,a high protein recovery rate of 85.23%and a COD removal rate of 73.32%was obtained.The NaCl of 0.006%introduced into the surimi rinsing liquid in this method does not increase the cost of subsequent wastewater treatment,and has a certain feasibility.It will provide a theoretical basis for recovering and utilizing sarcoplasmic protein in surimi rinsing water,and a technical support and reference for purifying rinsing wastewater during the process of surimi.