肠癌组织中miR-141的表达及其对HCT116细胞增殖和迁移的影响

The expression of microRNA-141 in colon cancer tissues and its effect on proliferation and metastasis of HCT116 cells

目的:探讨miR-141在肠癌组织中的表达情况以及其对HCT116细胞生物学功能的影响及其作用机制。方法:选取2016年05月至2018年05月在中国医科大学附属第一人民医院手术切除的30对肠癌组织以及癌旁组织进行miRNA芯片筛查。逆转录定量聚合酶链反应分析其中异常表达miRNA情况,评估miR-141的表达与肿瘤相关信息的相关性。通过TargetScan软件分析miR-141可能靶向的蛋白,在HCT116细胞中通过荧光素酶报告基因实验检测miR-141对DEK蛋白的靶向作用。HCT116细胞中分别过度表达和沉默表达miR-141后,通过MTT实验检测细胞增殖情况,Transwell实验检测细胞的迁移情况。结果:芯片分析和逆转录定量聚合酶链反应指出miR-141在肠癌组织中表达低于癌旁组织,miR-141与肿瘤的进程具有相关性,TargetScan软件指出miR-141可以靶向作用于DEK,荧光素酶报告基因实验印证了miR-141对DEK的靶向作用。MTT实验指出miR-141过度表达显著抑制细胞增殖,miR-141沉默表达显著促进细胞增殖,与对照组相比,差异有统计学意义(P<0.05)。Transwell实验显示,miR-141过度表达可以抑制HCT116细胞的迁移,miR-141沉默表达后HCT116细胞的迁移得到促进。结论:miR-141通过靶向DEK蛋白能够抑制HCT116细胞的增殖和迁移。

Objective:To investigate the expression of miR-141 in colon cancer and its effect on the biological function of HCT116 cells and its mechanism.Methods:30 cases of colon cancer and adjacent tissues were screened by microarray from May 2016 to May 2018 in the First People's Hospital Affiliated to China Medical University.Reverse transcription quantitative polymerase chain reaction(RT-qPCR)was used to analyze the abnormal expression of miRNAs,and to evaluate the correlation between the expression of miR-141 and tumor-related information.TargetScan software was used to analyze the possible targeting proteins of miR-141.Luciferase reporter gene assay was used to detect the targeting effect of miR-141 on DEK in HCT116 cells.After overexpression and silence of miR-141 in HCT116 cells,MTT assay was used to detect cell proliferation and Transwell assay was used to detect cell migration.Results:Microarray analysis and reverse transcription quantitative polymerase chain reaction indicated that the expression of miR-141 in colon cancer tissues was lower than that in adjacent tissues.miR-141 was related to the process of cancer.TargetScan software indicated that miR-141 could target DEK.Luciferase reporter gene experiment confirmed the targeting effect of miR-141 on DEK.MTT assay showed that miR-141 overexpression significantly inhibited cell proliferation,and miR-141 silent expression significantly promoted cell proliferation.There was a significant difference compared with the control group(P<0.05).Transwell assay showed that overexpression of miR-141 inhibited the migration of HCT116 cells,and the migration of HCT116 cells was promoted when miR-141 was inhibited.Conclusion:miR-141 can inhibit the proliferation and metastasis of HCT116 cells by targeting DEK.