白藜芦醇通过下调POLD1抑制乳腺癌MDA-MB-231细胞上皮间质转化

RSV Inhibits Epithelial-mesenchymal Transition of MDA-MB-231 Cells by Down-regulating POLD1 Expression

目的探讨白藜芦醇(RSV)通过下调POLD1对MDA-MB-231细胞上皮间质转化的影响。方法CCK-8检测RSV对MDA-MB-231细胞活性的抑制能力。使用重组慢病毒转染MDA-MB-231细胞构建POLD1-OE及POLD1-NC细胞系,RSV分别刺激各细胞后,蛋白印迹法检测POLD1、EMT相关蛋白E-cadherin、N-cadherin及Vimentin的表达;Transwell侵袭和划痕实验分别检测各实验组细胞的侵袭和迁移能力。结果RSV能显著抑制MDA-MB-231细胞的存活率,同时能够降低POLD1、N-cadherin及Vimentin的表达,上调E-cadherin的表达,且明显抑制细胞的迁移和侵袭能力;但上述作用效应在POLD1过表达的MDA-MB-231中被一定程度拮抗。结论RSV能够抑制MDA-MB-231细胞的活性及EMT,下调POLD1是RSV抑制MDA-MB-231细胞EMT的关键机制之一。

Objective To investigate the effect of resveratrol(RSV)on epithelial-mesenchymal transition of MDA-MB-231 cells by down-regulating POLD1 expression.Methods CCK-8 was used to detect the effect of RSV on the activity of MDA-MB-231 cells.POLD1-OE and POLD1-NC cell lines were constructed by transfecting MDA-MB-231 cells with recombinant lentivirus.Western blot was used to detect the expression of POLD1,E-cadherin,N-cadherin and Vimentin after RSV treatment.Transwell invasion experiment and the scratch test were used to detect the cells invasion and migration abilities of each experimental group.Results RSV could significantly inhibit the survival of MDA-MB-231 cells,reduce the expression of POLD1,N-cadherin and Vimentin,increase the expression of E-cadherin,and inhibit the abilities of cell invasion and migration.Increasing the POLD1 expression could reduce the above-mentioned biological effects of RSV on MDA-MB-231 cells.Conclusion RSV could significantly inhibit the viability and EMT of MDA-MB-231 cells by down-regulating the expression of POLD1.