腮腺多形性腺瘤及癌在多形性腺瘤中PINK1和Parkin的表达及临床意义

Expression and Significance of Mitochondrial Autophagy-associated Protein PINK1 and Parkin in Parotid Pleomorphic Adenoma and Carcinoma ex Pleomorphic Adenoma

目的探讨腮腺多形性腺瘤(PA)和癌在多形性腺瘤(CA-EX-PA)中线粒体自噬相关基因PINK1和Parkin的表达情况及其临床意义。方法免疫组织化学法检测24例腮腺正常组织标本、32例PA组织和42例CA-EX-PA组织中PINK1和Parkin的表达情况,并结合临床病理因素进行分析。结果PINK1蛋白在正常腮腺组织、PA组织和CA-EX-PA组织中的阳性率依次为100%、91%和67%;Parkin阳性表达率依次为100%、88%和52%;两者在PA和CA-EX-PA组织中的表达率显著低于正常组织(P<0.05)。PINK1和Parkin蛋白在CA-EX-PA组织中的表达具有正相关性(r=0.877,P<0.05)。在CA-EX-PA组织中,侵袭性、TNM分期和淋巴结转移与PINK1和Parkin的表达显著相关(P<0.05)。结论PINK1和Parkin在PA和CA-EX-PA组织中低表达,线粒体自噬活性降低在腮腺肿瘤的发生发展和侵袭转移中可能发挥一定的作用。

Objective To investigate the expression of mitochondrial autophagy-associated protein PINK1 and Parkin in parotid pleomorphic adenoma(PA)and carcinoma ex pleomorphic adenoma(CA-EX-PA).Methods The expression of PINK1 and Parkin were detected by immunohistochemistry in 24 cases of normal parotid gland tissues,32 cases of PA tissues and 42 cases of CA-EX-PA tissues.The correlations of PINK1 and Parkin expression with the clinicopathologic characteristics of CA-EX-PA patients were analyzed.Results The positive rates of PINK1 in normal parotid gland,PA and CA-EX-PA tissues were 100%,91%and 67%respectively;and those of Parkin were 100%,88%and 52%respectively.The expression rates of PINK1 and Parkin in PA and CA-EX-PA tissues were significantly lower than those in normal tissues(P<0.05).The expression of PINK1 and Parkin protein were positively correlated in CA-EX-PA tissues(r=0.877,P<0.01).In CA-EX-PA tissues,the expression of PINK1 and Parkin were associated with tumor invasion,lymph node metastasis and TNM stage(P<0.05).Conclusion The expression of PINK1 and Parkin are decreased in PA and CA-EX-PA tissues.The decrease of mitochondrial autophagy activity might play important roles in the development,invasion and metastasis of parotid gland tumors.