石斛多糖山楂复合饮料制备及缓解运动性疲劳的作用

Study on Preparation of Mixed Beverage of Dendrobium candidum Polysaccharide and Hawthorn and Its Effect on Relieving Exercise Fatigue

以铁皮石斛多糖和山楂汁为原料,研制石斛多糖山楂复合饮料,并观察其对机体运动性疲劳的缓解效果。在单因素实验的基础上,分别采用正交试验与响应面试验,确定复合饮料的组分最佳配比后,测定不同组别小鼠的力竭游泳运动时间和相关生化指标。结果表明,该复合饮料的最佳配方参数为:石斛多糖溶液与山楂汁的体积比为1:3、蔗糖用量4%、柠檬酸用量0.4%、羧甲基纤维素钠0.20%、海藻酸钠0.08%、黄原胶0.04%,制得的饮料呈浅黄绿色、色泽均一且透亮,酸甜适中,口感爽滑,具有铁皮石斛与山楂混合香味,静置后未有明显沉淀与分层,感官评分达到(91.7±0.4)分,离心沉淀率为0.61%±0.02%。与空白对照组相较,中、高剂量的石斛山楂复合饮料可分别延长小鼠力竭游泳时间1.5 min (P<0.05)和4.4 min (P<0.01),体内血乳酸浓度分别降低1.2 mmol/L(P<0.05)和3.4 mmol/L(P<0.01),血尿素氮浓度分别减小2.6 mmol/L(P<0.01)和3.5 mmol/L(P<0.01),因此该复合饮料有利于缓解机体运动性疲劳,可推广应用于运动营养领域。

A new formula of compound beverage was developed by polysaccharides of Dendrobium candidum and hawthorn juice as raw materials.On the basis of single factor tests,analyzing the optimum parameters of the mixed beverage byorthogonal test and response surface method,then measuring the exhaustive swimming time and related biochemical indexes in different groups of mice,respectively.Results showed that,the optimum parameters of mixed beverage were as follows:The volume ratio of polysaccharide extract of Dendrobium candidum and hawthorn juice was 1:3,the amount of sucrose was 4%,the amount of citric acid was 0.4%,the amount of sodium carboxymethyl cellulose was 0.20%,the amount of sodium alginate was 0.08%,and the amount of xanthan gum was 0.04%.The sensory evaluation score and centrifugal sedimentation rate of the beverage that optimum process preparation was(91.7±0.4)scores and 0.61%±0.02%.The beverage solution presented a light yellow-green color,transparent,and smooth taste with the proper acidity and sweet,without obvious precipitation or stratification after storage,and had strong mixed fragrance of Dendrobium candidum and hawthorn.Compared with the blank control group,the exhaustive swimming time of the groups of compound beverage at middle and high doses could separately prolong 1.5 min(P<0.05)and 4.4 min(P<0.01)in mice,the BLA concentration respectively decreased 1.2 mmol/L(P<0.05)and 3.4 mmol/L(P<0.01)in vivo,and BUN concentration separately decreased 2.6 mmol/L(P<0.01)and 3.5 mmol/L(P<0.01)in vivo.Therefore,the mixed beverage of Dendrobium candidum polysaccharide and hawthorn had better relieve exercise fatigue,and could be used in the field of sports nutrition.