杜仲叶中黄酮类化学成分研究

Study on Flavonoids from Leaves of Eucommia ulmoides

目的:研究杜仲Eucommia ulmoides叶的化学成分。方法:采用硅胶、聚酰胺、Sephadex LH-20和ODS柱色谱等方法对杜仲叶的化学成分进行分离纯化,并根据质谱、核磁等波谱数据进行结构鉴定。结果:从杜仲叶80%乙醇提取物中分离得到13个黄酮类化合物,鉴定为圣草素(eriodictyol,1)、柚皮素(naringenin,2)、山柰素(kaempferol,3)、洋芹素(apigenin,4)、山柰素-3-O-α-L-鼠李糖苷(kaempferol-3-O-α-L-rhamnoside,5)、紫云英苷(astragalin,6)、山柰素-3-O-α-L-吡喃阿拉伯糖苷(kaempferol-3-O-α-L-arapyranoside,7)、山柰素-3-O-β-D-6′-乙酰基吡喃葡糖苷(kaempferol-3-O-β-D-6′-acetylglucopyranoside,8)、槲皮素(quercetin,9)、槲皮素-3-O-α-L-鼠李糖苷(quercetin-3-O-α-L-rhamnoside,10)、槲皮素-3-O-β-D-吡喃葡萄糖基-β-D-吡喃木糖苷(quercetin-3-O-β-D-glucopyranosyl-β-D-xylopyranoside,11)、葛根素(puerarin,12)、大豆苷(daidzin,13)。结论:从杜仲叶中分离获得13个黄酮类化合物,其中化合物1、48、10、11均为首次从杜仲中分离得到。

Objective:To investigate the chemical constituents of leaves of Eucommia ulmoides.Methods:Silica,polyamide,Sephadex LH-20 and ODS column chromatographic techniques were used to isolate and purify the chemical constituents.MS and NMR was employed to elucidate their structures.Results:Thirteen known flavonoids were isolated from the 80%EtOH extract of leaves of E.ulmoides.Their structures were identified as eriodictyol(1),naringenin(2),kaempferol(3),apigenin(4),kaempferol-3-O-α-L-rhamnoside(5),astragalin(6),kaempferol-3-O-α-L-arapyranoside(7),kaempferol-3-O-β-D-6′-acetylglucopyranoside(8),quercetin(9),quercetin-3-O-α-L-rhamnoside(10),quercetin-3-O-β-D-glucopyranosyl-β-D-xylopyranoside(11),puerarin(12),daidzin(13).Conclusion:Compounds 1,4-8,10、11 are obtained from E.ulmoides for the first time.