UPLC同时测定通迪胶囊中8个活性成分的含量

Simultaneous Determination of Eight Constituents in Tongdi Capsules by UPLC

目的:建立超高效液相色谱法(UPLC)同时测定通迪胶囊中8个活性成分的含量。方法:采用UPLC同时测定通迪胶囊中三七皂苷R1、白花丹醌、去氢延胡索甲素、人参皂苷Rg1、人参皂苷Re、延胡索乙素、鸡矢藤苷甲酯、人参皂苷Rb1的含量,采用ACQUITY UPLC BEH C18色谱柱(150 mm×2.1 mm,1.7μm),流动相为乙腈(A)-0.03%磷酸溶液(B),梯度洗脱;检测波长203 nm(三七皂苷R1、人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1)、270 nm(白花丹醌及去氢延胡索甲素)、280 nm(延胡索乙素)、347 nm(鸡矢藤苷甲酯),流速0.3 mL·min^(-1),柱温30℃,进样量2μL。结果:三七皂苷R1、白花丹醌、去氢延胡索甲素、人参皂苷Rg1、人参皂苷Re、延胡索乙素、鸡矢藤苷甲酯、人参皂苷Rb1检测质量浓度分别为0.031~0.612、0.011~0.221、0.016~0.321、0.020~0.402、0.011~0.210、0.021~0.414、0.005~0.099、0.011~0.228 mg·mL^(-1);平均加样回收率分别为99.7%、98.4%、96.2%、99.6%、96.9%、95.7%、96.9%、97.4%。结论:该方法简单、有效、结果准确,可用于通迪胶囊中上述8个活性成分含量的同时测定。

Objective:To establish an UPLC method for the simultaneous determination of eight constituents in Tongdi Capsules.Methods:The determination was performed on ACQUITY UPLC BEH C18(150 mm×2.1 mm,1.7μm)column with mobile phase consisted of acetonitrile-0.03%phosphoric acid(gradient elution)at the flow rate of 0.3 mL·min-1.The detection wavelength was set at 203 nm for notoginsenoside R1,ginsenoside Rg1,ginsenoside Re and ginsenoside Rb1,270 nm for plumbagin and dehydrofusone,280 nm for tetrahydropalmatine,347 nm for feretoside,the column temperature was set at 30℃and the injection volume was 2μL.Results:The linear range was 0.031-0.612 mg·mL^(-1) for notoginsenoside R1,0.011-0.221 mg·mL^(-1) for plumbagin,0.016-0.321 mg·mL^(-1) for dehydrofusone,0.020-0.402 mg·mL^(-1) for ginsenoside Rg1,0.011-0.210 mg·mL^(-1) for ginsenoside Re,0.021-0.414 mg·mL^(-1) for tetrahydropalmatine,0.005-0.099 mg·mL^(-1) for feretoside,0.011-0.228 mg·mL^(-1) for ginsenoside Rb1,respectively.Average recoveries were 99.7%,98.4%,96.2%,99.6%,96.9%,95.7%,96.9%and 97.4%,respectively.Conclusion:The method is simple,effective and accurate.It can be used for simultaneous determination of 8 active constituents in Tongdi Capsules.