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中国襀翅目昆虫DNA条形码技术研究

DNA barcodes of Plecoptera from China
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摘要 DNA条形码技术在物种快速鉴定与种群遗传分化等方面发挥着重要作用,但目前基于DNA条形码技术针对中国襀翅目昆虫的研究尚未见报道.本研究利用生物信息学方法在基因数据库中获取中国襀翅目DNA条形码序列42条.通过碱基组成分析发现,条形码序列具有A和T碱基偏好性,同时具有较多的保守序列位点.基于DNA条形码序列的系统进化分析表明,DNA条形码在种级和属级水平具有较好的鉴定能力,为进一步利用DNA条形码技术全面研究中国襀翅目昆虫奠定了基础. DNA barcod plays an important role in species rapid identification and population genetic differentiation,but there is no report on the research of Chinese Plecoptera based on DNA barcode technology.In this study,42 DNA barcode sequences of Plecoptera from China were obtained with bioinformatic means.Base composition analysis revealed characteristics as A-T bias and multi conservative sites of the barcode sequences.Phylogenetic analysis supported the effective identification ability of DNA barcoding at species and genus level,which laid a foundation for further study of Plecoptera from China.
作者 陈志腾 CHEN Zhiteng(School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang 212100, China)
出处 《江苏科技大学学报(自然科学版)》 CAS 北大核心 2021年第2期99-104,共6页 Journal of Jiangsu University of Science and Technology:Natural Science Edition
基金 江苏科技大学科研启动基金资助项目(1182931901) 江苏省自然科学基金项目(BK20201009)。
关键词 襀翅目 DNA条形码 分子鉴定 生物多样性 Plecoptera DNA barcodes molecular identification biodiversity
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  • 1SHOMRON N, GOLAN D, HORNSTEIN E. An evolu- tionary perspective of animal microRNAs and their tar- gets [ J ]. Biomed Research International, 2009 ( 1 ) : 83 -99.
  • 2ZHANG G, HUSSAIN M, ONEILL S L, et al. Wolba- chia uses a host microRNA to regulate transcripts of a methyltransferase contributing to dengue virus inhibi- tion in Aedes aegypti [ J ]. Proceedings of the National Academy of Sciences, 2013, 110 (25): 10276 - 10281.
  • 3LINDBO J A, SILVA-ROSALES L, PROEBSTING W M, et al. Induction of a highly specific antiviral state in transgenic plants : implications for regulation of gene expression and virus resistance[ J]. Plant Cell, 1993, 5(5) : 1749 - 1759.
  • 4WU P, HAN S, CHEN T, et al. Involvement of mi- croRNAs in infection of silkworm with Bombyx mori cy- toplasmic polyhedrosis virus (BmCPV) [ J ]. PLOS One, 2013, 8(7) :e68209.
  • 5TRUJILLO R D, YUE S B, TANG Y, et al. The po- tential functions of primary microRNAs in target recog- nition and repression [ J]. Embo Journal, 2010, 29 (19) : 3272 - 3285.
  • 6LEE Y, AHN C, HAN J, et al. The nuclear RNase III Drosha initiates mieroRNA processing [ J ]. Nature, 2003, 425(6956): 415-419.
  • 7DU T, ZAMORE PD. mieroPrimer: the biogenesis and function of microRNA [J]. Development, 2005, 132 (21) : 4645 -4652.
  • 8GHILDIYAL M, XU J, SEITZ H, et al. Sorting of Drosophila small silencing RNAs partitions microRNA * strands into the RNA interference pathway [ J ]. RNA, 2010, 16(1) : 43 -56.
  • 9YANG J S, SMIBERT P, WESTHOLM J O, et al. In- tertwined pathways for Argonante-mediated microRNA biogenesis in Drosophila[ J]. Nucleic Acids Research, 2014, 42(3) : 1987 -2002.
  • 10OKAMURA K, HAGEN J W, DUAN H, et al. The mirtron pathway generates microRNA-class regulatory RNAs in Drosophila[J]. Cell, 2007, 130(1) : 89 - 100.

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