大鼠离体小肠吸收实验的改进

Improvement of Absorption Experiment of Isolated Small Intestine of Rats

大鼠离体小肠吸收实验是生物药剂学实验技术的重要组成部分,在本科实验教学中占有重要地位。实验改进前测得的数据波动范围大,误差大,重现性差,影响结果的准确性,因此从药物的选择、定量方法、酚红的影响等方面进行实验教学改革。改进后的大鼠离体小肠吸收实验操作简便,实验结果重现性及稳定性良好,有助于学生掌握药物吸收速率常数K_a的测定方法,计算有关药动学参数,加深对生物药剂学相关理论知识的理解,适合作为本科教学实验项目。

Absorption experiment of isolated small intestine of rats is an important part of experimental technology of biopharmaceutics,and it plays an important role in the undergraduate experimental teaching.Because of the large fluctuation range,large error and bad repeatability of the data measured before the experiment improvement,we reformed the experiment teaching from the choice of medicine,the quantitative method and the effect of phenol red.Results turned out that the improved experiment was simple and convenient,and the reproducibility and stability of the experimental results were good.It was helpful for students to master the determination method of drug absorption rate constant K_a,calculate related pharmacokinetic parameters,and deepen the understanding of the relevant theoretical knowledge of biopharmaceutics.In general,it is suitable for undergraduate teaching experiment.