摘要
白腐真菌锰过氧化物酶酶活性的测定方法很多,不同研究方法之间的锰过氧化物酶酶活性没有统一的衡量标准,无法进行有效的比较与分析。本试验选用白腐真菌一色齿毛菌,对锰过氧化物酶酶活性测定条件进行了研究,结果表明锰过氧化物酶酶活性最佳测定条件为柠檬酸-柠檬酸钠缓冲溶液(pH 3.5)0.1 mol/L,2,6-DMP 10 mmol/L,MnSO_(4)10 mmol/L,H_(2)O_(2)0.01 mol/L。
There are many methods to determine the activity of manganese peroxidase in white-rot fungi.There is no uniform standard for measuring the activity of manganese peroxidase among different studies,so it is impossible to compare and analyze it effectively.Cerrena unicolor was selected to study the conditions for the determination of manganese peroxidase activity.The results showed that the optimum conditions for the determination of manganese peroxidase activity were as follows:0.1 mol/L citric acid sodium citrate buffer solution(pH 3.5),10 mmol/L 2,6-DMP,10 mmol/L MnSO_(4),0.01 mol/L H_(2)O_(2).
作者
杨慧
武宸睿
陈桂东
张雅蕊
张会娟
尚洁
Yang Hui;Wu Chenrui;Chen Guidong;Zhang Yarui;Zhang Huijuan;Shang Jie(College of Biological Science and Engineering,North Minzu University,Yinchuan,Ningxia 750021)
出处
《宁夏农林科技》
2021年第2期67-70,共4页
Journal of Ningxia Agriculture and Forestry Science and Technology
基金
北方民族大学级大学生创新项目(S2019-11407-042)
北方民族大学科研项目(2018XYZSK05)。
关键词
一色齿毛菌
锰过氧化物酶
优化
Cerrena unicolor
Manganese peroxidase
Optimization