血清抗体检测在一起新型冠状病毒肺炎疫情传播链推断中的应用

Application of serum antibody detection in the identification of transmission chain of COVID-19

【目的】探讨血清抗体检测在新型冠状病毒肺炎(COVID-19)疫情传播链推断中的应用,为疫情防控提供科学依据。【方法】对确诊病例和密切接触者进行现场流行病学调查,采用实时逆转录聚合酶链反应(RT-PCR)法检测咽拭子、肛拭子标本新型冠状病毒核酸,采集血清进行IgM单独检测和IgM/IgG联合检测。【结果】确诊病例A无明确COVID-19流行病学史;病例C、D与A同餐同住,且与其他COVID-19确诊病例(H、L)有接触史和聚餐史。病例A新型冠状病毒核酸检测结果阳性,病例C和D均阴性。病例A和C单独IgM检测均阳性,病例D阴性;病例A、C和D IgMZIgG联合检测结果均阳性。病例C无临床症状,而病例D有临床症状。[结论]血清抗体检测可作为COVID-19传播证据链推断的有效补充,为找寻传染源和完善证据链提供参考依据。

[Objective]To explore the transmission chain of COVID-19 by serum antibody detection,and to provide scientific evidence for the prevention and control of the epidemic.[Methods]Field epidemiological investigation was used to determine the COVID-19 cases and their close contacts.The 2019-nCoV nucleic acid in throat swabs and anal swabs were examined by RT-PCR.Serum specimens were collected for anti-2019-nCoV IgM antibody detection and combined IgM/IgG detection.[Results]Case A had no confirmed exposure to COVID-19.However,case C and D had dinner and lived together with case A;they reported contact history and dinner history with other confirmed COVID-19 cases(H,L).Case A tested positive for 2019-nCoV nucleic acid,whereas case C and D were negative.Moreover,case A and C were IgM antibody positive,while case D was negative.Case A,C and D were all positive for combined IgM/IgG.In addition,case D had clinical symptom,while case C did not.[Conclusion]Serum antibody detection can be used as an effective supplement to the inference of transmission chain of COVID-19,which may facilitate detennining the source of infection and improving the evidence.