重离子束辐射对海带配子体克隆的影响

Effects of Heavy Ion Beam Radiation on the Gametophyte Clones of Saccharina Japonica

利用12C重离子束对东方海洋种质库保存的8个海带配子体克隆系进行3次辐射试验,辐射剂量分别为10、20、40、50、60、80、120、160、200和240 Gy。结果表明,使海带配子体克隆细胞发生变化的最低辐射剂量为60 Gy,随着剂量的增大,细胞死亡增多,240 Gy辐射剂量下仅有一个克隆系存活;80 Gy辐射剂量下细胞存活率约为50%~60%;不同海带配子体克隆系对重离子辐射的耐受度稍有差别;雄配子体比雌配子体耐受力稍强。

This study used 12C heavy ion beam to perform three radiation experiments on eight gametophyte clones of Saccharina japonica preserved in the germplasm bank of Shandong Oriental Ocean Sci-tech Co.Ltd..The radiation doses were 10 Gy、20 Gy、40 Gy、50 Gy、60 Gy、80 Gy、120 Gy、160 Gy、200 Gy、240 Gy.The results showed that the lowest radiation dose that changed the gametophyte clones of Saccharina japonica was 60 Gy.As the dose increased,the number of dead cell increased and only one gametophyte clone survived at 240 Gy dose.The survival rate was 50%-60%at 80 Gy radiation dose.There were slight differences in the tolerance to heavy ion radiation among gametophyte clones.The male gametophyte showed a slightly stronger tolerance than the female gametophyte.