A组P[15]型牛轮状病毒VP8*蛋白多糖结合特异性研究

Glycan binding specificity of VP8*protein of P[15]serotype bovine group A rotavirus

目的研究A组P[15]型轮状病毒VP8*蛋白的多糖结合特异性。方法利用大肠杆菌表达系统制备A组P[15]型牛轮状病毒毒株的VP8*蛋白,通过寡糖结合实验、生物膜层干涉实验及血凝等方法研究其与不同糖的结合。结果A组P[15]型牛轮状病毒VP8*蛋白结合Neu5Ac和Neu5Gc唾液酸,与α2,3和α2,6方式连接的唾液酸糖也有较好的结合。此外,P[15]VP8*蛋白可以凝集人和动物的红细胞。结论唾液酸可能是A组P[15]型轮状病毒的糖受体。与猴P[3]、猪P[7]轮状病毒相似,牛P[15]VP8*蛋白与唾液酸结合,并且序列和结构分析显示它们具有相同的糖结合位点。

Objective To investigate the glycan binding specificity of VP8*protein of P[15]group A rotavirus.Methods The VP8*protein of P[15]bovine group A rotavirus was obtained by the Escherichia coli expression system.The binding of P[15]VP8*protein to different glycans was tested by the assays of oligosaccharide binding,biolayer interferometry and hemagglutination.Results The VP8*protein of P[15]bovine group A rotavirus recognized sialic acids of Neu5Ac and Neu5Gc,and interacted with sialic acid linked to glycans byα2,3 andα2,6 linkage.Besides,bovine P[15]VP8*hemagglutinated different human and animal red blood cells.Conclusions Sialic acid may be the glycan receptor of P[15]bovine group A rotavirus.Similar to rhesus P[3]and porcine P[7]rotaviruses,bovine P[15]VP8*bound to sialic acid and possessed the same potential glycan binding site according to the sequence alignment and structural comparison.