全糯性小麦济糯116的籽粒蛋白质组学分析

Proteomic Analysis of Waxy Wheat Jinuo 116

糯小麦是指不含直链淀粉或者直链淀粉含量很低的小麦品种,它对小麦的淀粉以及面团等特性都有很大的影响。解释小麦淀粉糯性变异的机制,对于改良小麦品质具有重大意义。本研究以全糯性小麦济糯116及普通小麦济麦22为材料,利用TMT(tandem mass tags)定量蛋白质组学技术结合生物信息学分析法,分析差异表达蛋白及其功能和通路等富集情况。结果表明,两个小麦品种中共鉴定出161个差异表达蛋白,其中上调蛋白59个、下调蛋白102个。差异蛋白的GO富集分析发现,在分子功能中,富集在淀粉合成酶活性类、核糖体RNA N-糖基化酶活性类和RNA糖基化酶活性类蛋白的差异倍数较大。KEGG分析发现,核糖体代谢途径及淀粉和蔗糖代谢途径中的差异蛋白最多,表明除淀粉和蔗糖代谢外,核糖体代谢也可能参与小麦糯性机制。本研究为进一步探索小麦籽粒糯性遗传机制提供了参考。

Waxy wheat is a variety of wheat with no or very low amylose,which has a great effect on the starch and dough properties.It is crucial to understand the genetic basis of glutinous variation for improving wheat quality.In this study,the concentration of differentially expressed proteins(DEPs)and their functions and pathways of waxy wheat Jinuo 116 and common wheat Jimai 22 were analyzed using tandem mass tags(TMT)quantitative proteomics and bioinformatics analyses.A total of 161 DEPs were identified between the two wheat cultivars,including 59 up-regulated proteins and 102 down-regulated proteins.The GO enrichment analysis of the DEPs showed that a large difference in the proteins enriched in starch synthetase activity,ribosomal RNA N-glycosylase activity and RNA glycosylase activity in the molecular function.KEGG analysis demonstrated that there were the most as 10 and 8 DEGs in the ribosome metabolic pathway and starch and sucrose metabolic pathway,respectively.Ribosome metabolism might also be involved in the glutinous mechanism of wheat in addition to starch and sucrose metabolism.This study provided references for further exploring the genetic mechanism of glutinous in wheat.