基于年龄相关DNA甲基化位点推断河南汉族个体年龄

Applicability of Age-related DNA Methylation Sites to Infer Ages of Han-ethnic Individuals in Henan Province

目的探查中国河南汉族个体与年龄相关的DNA甲基化位点,构建年龄推断模型,进行甲基化和年龄相关性分析。方法采用焦磷酸测序法对ELOVL2、ClOrf132、KLF14、TRIM59和FHL2基因的34个CG位点进行甲基化分析,利用SPSS 23软件的多元回归方法建立模型,对甲基化和年龄相关性做分析。结果除ClOrf132基因3个CG位点的甲基化水平与年龄呈负相关外,其余4个基因的31个CG位点甲基化水平均与年龄呈正相关。多元回归分析表明,年龄与CG位点的甲基化水平存在明显的线性关系,实际年龄与推断年龄偏差在5岁以内的准确度达80%以上。结论本研究构建的河南汉族个体年龄推断模型,有助于通过检测血液等组织的DNA甲基化水平推断个体的年龄范围,具有法医学应用前景。

Objective To survey the age-related DNA methylation sites in Chinese Han-ethnic population so as to construct an age inference model for analyzing the correlation between DNA methylation and age.Methods 34 CpG loci of ELOVL2,ClOrf132,KLF14,TRIM59 and FHL2 genes were analyzed into their DNA methylation through pyro-sequencing.The resulted data were processed with SPSS23 multiple regression approach so that a relevant model was established for correlative analysis of DNA methylation and age.Results The methylation level of 31 CpG loci of four genes(ELOVL2,KLF14,TRIM59 and FHL2)was of positive correlation with age,with the other three CpG loci of ClOrf132 gene showing negative for the methylation-age correlation.Multiple regression analysis revealed that there was a linear relationship between age and methylation level,demonstrating an accuracy of more than 80%within deviation of 5 years old between actual age and the inferred.Conclusion The age inference model here-constructed of China’s Henan Han-ethnic population is able to help determine the age range of individuals by way of detecting the DNA methylation level of tissues(e.g.,the blood)such that the application prospects could be expected in forensic medicine.