HPLC法测定蒙成药三子散中没食子酸的含量

Determination of gallic acid in Mongolian patent medicine Sanzi powder by HPLC

目的:建立蒙成药三子散中没食子酸的含量测定方法。方法:采用HPLC法,采用Phenomenex C18色谱柱(250mm×4.6 mm,5μm),流动相为甲醇-0.1%磷酸水溶液(5∶95),流速1.0 mL(min^(-1)),柱温35℃,进样量为10μL,检测波长为273nm。结果:没食子酸进样量范围在6.357~158.935μg时,进样量与峰面积线性关系良好(r=0.9999);平均回收率为97.70%,RSD为1.30%。结论:该方法简便、灵敏、准确,可用于三子散中没食子酸的测定。

Objective:To establish a method for the determination of gallic acid in Mongolian patent medicine Sanzi powder.Methods:The HPLC method was established on a phenomenex C18 column(250 mm×4.6 mm,5μm),the mobile phase was methanol-0.1%phosphoric acid(5:95),the flow rate was 1.0 ml(min^(-1)),the column temperature was 35℃,the injection volume was 10μL,and the detection wavelength was 273nm.Results:The injection volume and peak area of gallic acid had a good linear relationship when the injection volume ranged from 6.357μg to 158.935μg(r=0.999).the average recovery was 97.70%,with RSD was 1.30%.Conclusion:This established mothod is simple,sensitive and accurate,and can be used for the determination of gallic acid in Sanzi powder.