超声辅助酶法提取无籽刺梨渣膳食纤维及理化性质评价

Ultrasonic Assisted Enzymatic Extraction of Dietary Fiber from Rosa Sterilis Pomace and Its Physicochemical Properties

采用超声辅助酶法提取无籽刺梨渣可溶性膳食纤维(RSDF)和不溶性膳食纤维(RIDF),以RSDF/RIDF比值为评价指标,研究了料液比、纤维素酶添加量、超声温度、超声功率、超声时间5个因素对RSDF/RIDF的影响,并采用响应面试验优化提取工艺,分析了脱脂刺梨粉和膳食纤维的理化性质。结果表明:料液比1:20 g/mL、纤维素酶添加量9%、超声温度50℃、超声功率250 W、超声时间40 min时,RSDF/RIDF比值最高为0.638±0.012,与理论预测值0.649相比,误差为0.011,表明响应面模型可靠性高。RTDF_(1:2)膨胀力、持水力分别为2.761 mL/g和3.248 g/g,显著高于脱脂刺梨粉和RIDF(P<0.05);持油力为2.603 g/g,显著高于脱脂刺梨粉和RSDF(P<0.05);溶解性为37.643%,显著高于RIDF(P<0.05)。相较于单一的RSDF或RIDF,RTDF_(1:2)理化性质得到综合提升和改善,表明RTDF_(1:2)具有良好的膳食纤维特性。

In the study,the soluble dietary fiber (RSDF) and insoluble dietary fiber (RIDF) from Rosa sterilis pomace was extracted by ultrasonic assisted enzymatic extraction technology.The effects of liquid-material ratio,cellulase dosage,ultrasonic temperature,ultrasonic power and ultrasonic time on the RSDF/RIDF from Rosa sterilis pomace were investigated by single factor experiments.On the basis of single factor experiment,extraction conditions were optimized by response surface methodology and the physicochemical properties were studied.The results showed that the optimum extraction conditions were:solid-liquid ratio 1:20 g/mL,cellulase dosage 9%,ultrasonic temperature 50 ℃,ultrasonic power 250 W,with ultrasonic time 40 min.Under these conditions,the RSDF/RIDF ratio was 0.638±0.012,and the theoretical prediction value was 0.649.The relative error was about 0.011.The optimized response surface equation was useful.The results of physicochemical properties showed that the swelling power and water holding capacity of RTDF_(1:2) were 2.761 mL/g and 3.248 g/g,respectively.Which were significantly higher than those of defatted Rosa sterilis pomace and RIDF (P<0.05).The oil holding capacity of RTDF_(1:2) was 2.603 g/g,significantly higher than those of defatted Rosa sterilis pomace and RSDF (P<0.05).And the water solubility was 37.643%,significantly higher than RIDF (P<0.05).Compared with a single RSDF or RIDF,the physicochemical properties of RTDF_(1:2) were improved.In conclusion,the total dietary fiber of from Rosa sterilis pomace is suitable as an additive used in health food with its good dietary fiber characteristics.