胞红蛋白调控血管平滑肌表型转换与高血压血管重建机制研究

A study of cytoglobin control on the vascular smooth muscle cell phenotype transition and hypertension-induced vascular remodeling mechanism

目的观察胞红蛋白调控血管平滑肌表型转换与高血压血管重建机制研究。方法选择我院实验中心饲养12周龄雄性SD大鼠30只,体质量(200±20)g。分为3组:肾动脉缩窄高血压药物组、肾动脉缩窄高血压组、正常对照组;高血压药物组大鼠用血管紧张素Ⅱ一型受体(AT1R)拮抗剂阿利沙坦酯灌胃,免疫荧光定量PCR法及免疫印迹检测Cygb表达水平,观察三组大鼠Anxa8、Tpm3、Retn1b mRNA基因表达变化;观察正常血压主动脉与高血压主动脉大鼠模型术后6周血管重建后Cygb mRNA表达水平。结果肾动脉缩窄高血压药物组、肾动脉缩窄高血压组的Anxa8、Tpm3基因表达显著高于正常对照组,Rentn1b基因表达显著降低(P<0.05);而肾动脉缩窄高血压药物组的Anxa8、Tpm3基因表达显著低于肾动脉缩窄高血压组,而Ren1b基因表达显著高于肾动脉缩窄高血压组(P<0.05);肾性高血压大鼠模型术后六周血管重建后Cygb的表达差异初步研究,发现与正常血压主动脉组比较,高血压大动脉中Cygb的mRNA表达下降(P<0.05);肾动脉缩窄高血压药物组、肾动脉缩窄高血压组的Cygb显著低于正常对照组,颈动脉中膜厚度显著高于正常对照组(P<0.05);肾动脉缩窄高血压药物组的Cygb显著高于肾动脉缩窄高血压组,而颈动脉中膜厚度显著低于肾动脉缩窄高血压组(P<0.05)。结论高血压胞红蛋白表达下降,在高血压血管重建机制中是有益的血管保护因子,可为高血压治疗潜在靶点研究提供生物学根据。

Objective To study the cytoglobin(Cygb)control on the vascular smooth muscle cell phenotype transition and hypertension-induced vascular remodeling mechanism.Methods 30 male SD rats(aged 12 weeks)bred in our experimental center from January 2019 to June 2020 were selected.Rats were divided into antihypertensive drug group,hypertensive group and control group.The antihypertensive drug group took the gastric administration with angiotensin II type 1 receptor(AT1R)antagonist Allisartan Isoproxil;Cygb expressions were measured by the fluorescence quantitative polymerase chain reaction(PCR)and immunoblotting method.Anxa8,Tpm3 and Retn1b mRNA gene expression changes were observed;after 6 weeks of vascular remodeling,Cygb mRNA expressions for rat models with normal aorta and hypertension-associated aorta were compared.Results Anxa8 and Tpm3 gene expressions in antihypertensive drug group and hypertensive group were significantly higher than control group while Rentn1b gene expressions were significantly lower than control group(P<0.05);Anxa8 and Tpm3 gene expressions in antihypertensive drug group were significantly lower than hypertensive group while Ren1b gene expressions were significantly higher than hypertensive group(P<0.05);the pilot research findings indicated that Cygb mRNA expressions of hypertension-associated aorta were remarkably declined by comparison to normal aorta after 6 weeks of vascular remodeling(P<0.05);Cygb expressions in antihypertensive drug group and hypertensive group were significantly lower than control group while carotid artery intima-media thickness was higher than control group(P<0.05);Cygb expressions in antihypertensive drug group were significantly higher than hypertensive group while carotid artery intima-media thickness was lower than hypertensive group(P<0.05).Conclusion Cygb expressions are declined in hypertension cases;however,the vascular remodeling is to offer the blood vessel protection factors,providing biological basis on the potential research of hypertension treatment.