氮掺杂石墨烯量子点/Ru(phen)_(3)^(2+)杂化体系电致化学发光法测定诺氟沙星

Study of sensitive electrochemiluminescence determination of norfloxacin based on nitrogen-doped graphene quantum dots/Ru( phen)_(3)^(2+) hybrid platform

制备了氮掺杂石墨烯量子点(NGQDs)并表征了其形貌和结构。利用诺氟沙星(NFX)充当主发光体Ru(phen)_(3)^(2+)共反应剂的条件下,NGQDs能够促进其自身与受体Ru(phen)_(3)^(2+)之间的共振能量转移并催化Ru(phen)_(3)^(2+)与NFX之间的电极反应,从而导致体系电致化学发光(ECL)信号显著增强的效果,构建了测定NFX的NGQDs/Ru(phen)_(3)^(2+)杂化新体系。体系ECL增量的对数值与NFX的浓度在1.0×10^(-8)~1.0×10^(-4)mol/L之间呈良好的线性关系,检测限为3.3 nmol/L。该方法可用于两种商业药品制剂中NFX的测定。

Nitrogen-doped graphene quantum dots( NGQDs) with high biocompatibility and good stability were prepared by a simple method and characterized by different techniques. In the presence of norfloxacin( NFX) as a co-reactant of the main luminophore Ru( phen)_(3)^(2+),NGQDs can promote the resonance energy transfer from excited donors( NGQDs) to acceptors( Ru( phen)_(3)^(2+)) and catalyze electrode surface reaction between Ru( phen)_(3)^(2+)and norfloxacin,resulting in a strong CL signal enhancement. Then NGQDs/Ru( phen)_(3)^(2+)hybrid platform was used to establish a novel electrochemiluminescence( ECL) sensor for the determination of NFX.Under the optimum experimental conditions,the linear calibration curve between logarithm of ΔECL and concentration of NFX in the range from 1. 0 × 10^(-8) to 1. 0 × 10^(-4) mol/L was obtained with the detection limit of 3. 3 nmol/L. Moreover,the analytical performances,such as selectivity,reproducibility and stability of the proposed method were also evaluated in detail. At the end,the NGQDs/Ru( phen)_(3)^(2+)hybrid platform was utilized for measuring the content of NFX in two types of pharmaceutic formulations with satisfactory results.Therefore,the proposed ECL system with the outstanding ECL efficiency may show a great potential in drug monitoring and environmental analysis.