孕酮分子印迹表面等离子共振传感器的制备

Molecularly imprinted film combined with surface plasmon resonance for the determination of progesterone

以孕酮为模板分子,甲基丙烯酸为单体,乙二醇二甲基丙烯酸酯为交联剂,二苯甲酮为光引发剂,通过现场光接枝的方法合成孕酮分子印迹膜(MIF)。原子力显微镜结果显示,MIF表面分布许多纳米尺寸的用于吸附模板分子的孔穴;采用表面等离子共振技术对1.0~1.0×10^(3)pmol/L的孕酮进行吸附检测,光强增加值与孕酮浓度的对数值间呈线性关系,检出限为0.3 pmol/L。7次洗脱-重新吸附后,MIF仍具有较好的传感性能,放置90 d后,孕酮分子印迹膜仍能进行很好洗脱和吸附。此分子印迹膜传感器用于人工尿液中检测孕酮,回收率在96.0%~102.5%之间。

By using progesterone as template molecule,methacrylic acid(MAA)as monomer,ethylene glycol dimethacrylate(EGDMA)as cross-linking agent and benzophenone(BP)as photo-initiator,the molecularly imprinted film(MIF)was synthesized by in-situ photo-grafting.The chip surface modified with aprogesterone-imprinted film was characterized by atomic force microscopy(AFM),showing that many cavities at nanometer scales were dispersed on the surface of MIF.By using surface plasmon resonance technology,a good linear relationship was obtained between the reflectivity and progesterone concentrations arranging from 1.0 to 1.0×10^(3) pmol/L,and the detection limit was 0.3 pmol/L.The MIF had good selectivity for template molecules.The MIF sensor could be regenerated 7 times of rinsing and re-adsorption without significant deterioration of the sensor performance.After placed for 90 days,the MIF still performed good rinsing and adsorption,indicating that it had good repeatability and stability.Progesterone was detected in artificial urine with recoveries in the range of 96.0%-102.5%.