甘肃省武威市胃癌患者肠道菌群分布

Distribution characteristics of intestinal flora in patients with gastric cancer in Wuwei,Gansu Province

目的通过比较甘肃省武威市胃癌患者和健康对照人群肠道菌群的分布,探讨胃癌患者肠道菌群的变化与胃癌发生发展的关系,并寻找可能作为该地区胃癌患者的潜在生物标志物。方法收集24例胃癌患者和24例健康对照人群的粪便样本,提取DNA,采用16S rRNA基因高通量测序进行肠道菌群分析。结果分析2组研究对象肠道菌群的Alpha多样性,发现胃癌患者组和健康对照组肠道菌群的Chao1指数差异无统计学意义(Z=-0.907,P=0.364),胃癌患者组的Simpson指数升高且差异具有统计学意义(Z=-3.258,P=0.001),表明武威市胃癌患者肠道菌群多样性增加;物种差异分析表明,2组人群在门水平上,Fusobacteria、Patescibacteria、Synergistetes的相对丰度变化差异具有统计学意义(均P<0.05),在属水平上,Bacteroides(拟杆菌属)、LachnospiraceaeUCG-008、Lachnoclostridium、Blautia、Roseburia等34个菌属的相对丰度变化差异具有统计学意义(均P<0.05)。通过菌属微生物关联网络图发现,在34个差异菌属中,Streptococcus、ErysipelotrichaceaeUCG-003等菌属的节性重要性较高;通过LEfSe分析和组间秩和检验分析发现,LacLachnospira、LachnospiraceaeUCG-004在健康对照组和胃癌患者组之间是显著差异的菌属。结论与健康对照组人群的肠道菌群比较,胃癌患者组的菌群多样性增加,胃癌患者肠道菌群的改变主要以Lachnospiraceae等益生菌属的相对丰度减少和Alloprevotella、Desulfovibrio等致病菌属的相对丰度增加为主。LacLachnospira、LachnospiraceaeUCG-004可能为武威市胃癌患者潜在生物标志物。

Objective To explore the relationship between the changes of intestinal flora and the occurrence and development of gastric cancer(GC), and find out the bacteria that may be used as potential biomarkers. Methods Fecal samples from 24 patients with GC and 24 healthy controls were collected. DNA was extracted and 16 S rRNA gene high-throughput sequencing was used to observe intestinal flora, and bioinformatics analysis was performed. Results By analyzing the Alpha diversity of intestinal microflora, there was no significant difference in Chao1 index between the patients and healthy controls(Z=-0.907, P=0.364);The Simpson index of patients with GC increased and had statistical significance(Z=-3.258, P=0.001), which indicated that the diversity of intestinal microflora in patients with GC increased in Wuwei city. Species difference analysis showed that there were significant differences in the relative abundances of Fusobacteria, Patescibacteria and Synergistetes between the two groups at phylum level(all P<0.05), and in those of 34 genera such as Bacteroides, LachnospiraceaeUCG-008, Lachnoclostridium, Blautia and Roseburia at genus level(all P<0.05). Through the association network map of bacteria and microorganisms, it was found that among the 34 different genera, Streptococcus, ErysipelotrichaceaeUCG-003 and other bacteria had higher nodular importance, and through LEfSe analysis and inter-group rank sum test, it was found that LacLachnospira and LachnospiraceaeUCG-004 changed significantly between patients with GC and healthy controls. Conclusion Compared with healthy people, the diversity of intestinal flora in patients with GC increased. The main changes of intestinal microflora in patients with GC are the decrease of relative abundance of probiotics such as Lachnospiraceae and the increase of relative abundance of pathogenic bacteria such as Alloprevotella and Desulfovibrio. LacLachnospira and LachnospiraceaeUCG-004 bacteria may be potential biomarkers for patients with GC in Wuwei city.