高盐溶液预处理幽门螺杆菌对蒙古沙土鼠胃黏膜损伤作用

Effects of Helicobacter pylori pretreated with 30% NaCl solution on gastric mucosal injury in Mongolian gerbils

目的探讨高盐预处理的幽门螺杆菌(H.pylori)对胃黏膜的损伤作用。方法将30%高盐预处理前后的胃癌来源的H.pylori菌株(4854)灌胃蒙古沙土鼠(MGs),在灌胃后13、26和73周解剖动物,通过组织病理学检查、免疫组化染色和黏膜厚度测量,探讨高盐预处理的H.pylori对胃黏膜的损伤作用。结果与未加盐预处理的相应菌株相比,高盐预处理组小鼠的慢性炎症、黏膜变性/坏死、腺体萎缩伴肠上皮化生的发生率较低,黏膜糜烂/溃疡和黏膜上皮增生的发生率较高,差异均有统计学意义(t=8.325 6,P=0.040 8)。第73周,高盐预处理4854菌株组胃体和胃窦黏膜增生显著高于未加盐预处理组(t=12.802 4,P=0.035 1;t=16.536 0,P=0.043 8)。结论高盐预处理改变了H.pylori的体内致病性,有助于阐明H.pylori感染与高盐饮食在胃病中的相互作用模式。

Objective To observe the damaging effect of H.pylori pretreated with high-salt solution on gastric mucosa. Methods Mongolian gerbils(MGs) were administered intragastrically gastric cancer-derived H.pylori strains(4854) with and without pretreatment of 30% high salt solution, respectively. The animals were dissected at 13, 26 and 73 weeks after the intragastrically administration. By using histopathology, immunohistochemical staining and mucosal thickness measurement, the salt-pretreated H.pylori-induced gastric mucosa injury was observed. Results The incidence of chronic inflammation, mucosal degeneration/necrosis, gland atrophy and intestinal metaplasia was lower, while that of mucosal erosion/ulcer and epithelial hyperplasia was significantly higher in the high salt pretreatment group than that in the control group, respectively(t=8.325 6, P=0.040 8). At the 73 rd week, the proliferation of gastric body and antrum mucosa in the salt pretreated group was significantly higher than that in the control group(t=12.802 4, P=0.035 1;t=16.536 0, P=0.043 8). Conclusion High salt pretreatment changed the pathogenicity of H.pylori in vivo, which is helpful to clarify the interaction between H.pylori infection and high salt diet in gastric diseases.