基于聚集诱导发光的甲醛荧光探针

Fluorescence Probe for Formaldehyde Based on Aggregation-induced Emission

甲醛能使DNA受损,引起肝肾功能障碍,并最终导致恶性肿瘤,被世界卫生组织确定为一类致癌物,因此,非常有必要开发能快速、方便检测甲醛的方法。本研究通过缩合4-二苯胺基苯甲醛与对硝基苯乙腈,再还原硝基合成了一个能选择性检测甲醛的荧光探针(PAN)。PAN具有聚集诱导发光性能,在含水体积分数为80%以上的乙腈/水中能产生明亮的绿色荧光。在含水体积分数为70%的乙腈/水中,PAN的荧光很弱,加入甲醛能迅速发生胺醛缩合反应并产生聚集诱导荧光,发出明亮的绿色荧光。其它所选7种常见含活性羰基的化合物都不能引起探针的发射光谱发生明显变化,总之,该探针具有较好的灵敏性、选择性,且具有一定的实用价值。

Formaldehyde can cause DNA damage,which induces liver and kidney dysfunction and eventually leads to cancers.It has been categorized as a class I carcinogen by the World Health Organization.Thus,the fast and convenient methods for detecting formaldehyde are significant for public health.Herein,a fluorescent probe(PAN)for formaldehyde was developed by incorporating 4-(diphenylamino)benzaldehyde and 4-nitrophenylacetonitrile and then reducing the nitro-group.The probe exhibits aggregation-induced emission enhancement(AIEE)characteristics and can produce bight green fluorescence in an acetonitrile/water mixture when the volume fraction of water is over 80%.In acetonitrile/water mixture when the volume fraction of water is 70%,PAN is well dispersed and emits weak fluorescence,but the addition of formaldehyde gives anaggregation-induced emission enhancement.The other 7 selected active carbonyl compounds did not cause noticeable fluorescence variations.In brief,PAN shows good sensitivity,selectivity and practicality.