LINC01088对胶质瘤U251细胞的增殖、迁移以及血管拟态的影响

Effects of LINC01088 on proliferation,migration,and angiogenesis of glioma U251 cells

目的探讨LINC01088对胶质瘤U251细胞的增殖、迁移、侵袭以及血管形成的影响。方法利用GEPIA网站分析LINC01088在胶质瘤组织中的表达。构建LINC01088沉默细胞稳定细胞株,MTT、Transwell、血管形成实验检测沉默LINC01088表达对U251细胞增殖、迁移和侵袭、血管生成影响。Western blot检测PI3K、p-AKT和AKT蛋白表达的影响。结果GEPIA分析结果显示,与正常组织相比,胶质瘤组织中的LINC01088相对表达水平显著上调(P<0.05)。转染sh-LINC01088稳定下调U251细胞中LINC01088的表达(P<0.05),并获得稳定低表达LINC01088的U251细胞株。与对照组相比,LINC01088沉默组细胞增殖、迁移和侵袭细胞、血管生成数量以及PI3K和p-AKT蛋白表达显著下降(P<0.05)。结论沉默LINC01088表达抑制U251细胞的增殖、迁移、侵袭和血管生成,其作用机制可能与抑制PI3K/AKT信号通路有关。

Objective To investigate the effects of LINC01088 on proliferation,migration,invasion and angiogenesis of glioma U251 cells.Methods The expression of LINC01088 in glioma was analyzed by GEPIA.The shRNA-LINC01088(sh-LINC01088)was used to infect U251 cells to establish the stable cell line.The effect of silencing LINC01088 on the proliferation,migration and invasion,angiogenesis of U251 cells were detected by MTT,Transwell,tube formation,respectively.The expression of PI3K,p-AKT and AKT in U251 cells was measured by Western blot.Results GEPIA analysis showed that the relative expression level of LINC01088 in glioma tissues was significantly higher than that in normal tissues(P<0.05).Transfection of sh-LINC01088 could down-regulate the expression of LINC01088 in U251 cells(P<0.05),and U251 cell lines with stable and low expression of LINC01088 was obtained.Compared with the control group,the proliferation,the cell number of migration and invasion,the numbers of tube,and the expression of PI3K and p-AKT in the sh-LINC01088 group were significantly decreased(P<0.05).Conclusion Silencing LINC01088 inhibits proliferation,migration,invasion and angiogenesis of U251 cells,and its mechanism might be related to the inhibition of PI3 K/AKT signaling pathway.