FOXK1、SATB2在胃癌组织中的表达及其与临床病理参数及预后的相关性分析

Expression of FOXK1 and SATB2 in Gastric Cancer Tissues and Their Correlation with Clinicopathological Parameters and Prognosis

目的探讨叉头框转录因子1(FOXK1)、特异AT序列结合蛋白2(SATB2)在胃癌组织中的表达及其与临床病理参数及预后的相关性。方法以153例胃癌患者为研究对象,应用PT-PCR技术检测FOXK1、SATB2在胃癌组织及癌旁正常组织中的表达,比较胃癌组织及癌旁组织中FOXK1、SATB2表达水平,采用ROC曲线分析SATB2、SATB2表达水平对胃癌的诊断价值;收集患者临床资料,分析胃癌患者FOXK1、SATB2表达与临床病理参数的关系,并采用多元logistics回归分析影响胃癌患者FOXK1、SATB2表达的危险因素;分析胃癌患者FOXK1、SATB2表达与预后的关系分析,并绘制生存曲线。结果胃癌组织中FOXK1、SATB2阳性表达率均高于癌旁组织(P<0.05);FOXK1、SATB2表达联合检测诊断胃癌的AUC大于单独检测(P<0.05);FOXK1阳性在肿瘤大小≥5 cm、淋巴结转移、TNM分期为Ⅲ、Ⅳ期、分化程度高方面的例数比例高于FOXK1阴性组,SATB2阳性在肿瘤大小≥5 cm、淋巴结转移、分化程度高方面的例数比例高于SATB2阴性组(P<0.05);肿瘤大小≥5 cm、淋巴结转移、TNM分期为Ⅲ、Ⅳ期、分化程度高是影响FOXK1表达的危险因素,肿瘤大小≥5 cm、淋巴结转移、分化程度高是影响SATB2表达的危险因素(P<0.05);FOXK1阳性组生存率低于FOXK1阴性组,SATB2阳性组生存率低于SATB2阴性组(P<0.05)。结论胃癌组织的FOXK1、SATB2阳性表达率均较高,肿瘤大小≥5 cm、淋巴结转移、TNM分期为Ⅲ、Ⅳ期、分化程度高是影响胃癌组织FOXK1、SATB2的危险因素,并且FOXK1、SATB2阳性表达患者预后较差。

Objective To explore the expression of forkhead box k1(FOXK1)and special AT-rich sequence-binding protein 2(SATB2)in gastric cancer tissues and their correlation with clinicopathological parameters and prognosis.Methods 153 gastric cancer patients were enrolled as the research objects.The expression of FOXK1 and SATB2 in gastric cancer tissues and para-carcinoma normal tissues was detected by PT-PCR.The expression levels of FOXK1 and SATB2 in gastric cancer tissues and para-carcinoma tissues were compared.The diagnostic value of FOXK1 and SATB2 for gastric cancer was analyzed by ROC curves.The clinical data of patients were collected.The relationship between FOXK1,SATB2 and clinicopathological parameters was analyzed.The risk factors that effected the expression of FOXK1 and SATB2 were analyzed by multivariate Logistics regression analysis.The relationship between FOXK1,SATB2 and prognosis in gastric cancer patients was analyzed.The survival curves were drawn.Results The positive expression rates of FOXK1 and SATB2 in gastric cancer tissues were higher than those in para-carcinoma tissues(P<0.05).AUC of FOXK1 combined with SATB2 for the diagnosis of gastric cancer was greater than that of single index(P<0.05).The proportions of cases with tumor≥5 cm,lymph nodes metastasis,TNM staging at stageⅢandⅣ,and high differentiation in FOXK1 positive group were higher than those in FOXK1 negative group.The proportion of cases with tumor≥5 cm,lymph nodes metastasis and high differentiation in SATB2 positive group were higher than those in SATB2 negative group(P<0.05).The tumor≥5 cm,lymph nodes metastasis,TNM staging at stageⅢandⅣ,and high differentiation were risk factors that effected FOXK1 expression,while tumor≥5 cm,lymph nodes metastasis and high differentiation were risk factors that effected SATB2 expression(P<0.05).The survival rate in FOXK1 positive group was lower than that in FOXK1 negative group,which was lower in SATB2 positive group than SATB2 negative group(P<0.05).Conclusion The positive expression rates of FOXK1 and SATB2 are higher in gastric cancer tissues.Tumor≥5 cm,lymph nodes metastasis,TNM staging at stageⅢandⅣ,and high differentiation are risk factors that affect FOXK1 and SATB2 in gastric cancer tissues.The prognosis is worse in patients with positive expression of FOXK1 and SATB2.