摘要
目的:研究口腔白斑发生发展中的血管生成基因表达谱,并验证关键基因表达。方法:对正常黏膜(n=3),低危白斑(n=4),高危白斑(n=4),早期鳞癌(n=6)进行转录组学芯片检测生物学作用为血管生成的相关基因,对4个关键基因表达进行了验证。结果:从高危白斑发展到鳞癌有151个基因,为全部异常表达基因占比18.9%。RT-qPCR验证结果显示VEGFA、PDGFRB、MMP1和MMP3在正常黏膜、口腔白斑和鳞癌中的表达量均呈阶梯式升高,与芯片结果基本一致。结论:血管生成基因的异常表达与白斑癌变关系密切。
Objective:To study the expression profile of angiogenic genes in the development of oral leucoplakia(OLK)and to verify the expression of key genes.Methods:The angiogenic genes of OLK in normal mucosa(n=3),low risk OLK(n=4),high risk OLK(n=4)and early OSCC(n=6)samples were detected by transeriprome microarray and the expression of 4 key genes were verified by RT-qPCR.Results:There were 151 differentially expressed genes from high risk OLK to OSCC,accounted for 18.9%of all abnormal genes.qRT-PCR results showed that the expression levels of VEGFA,PDGFRB,MMP1 and MMP3 in normal mucosa,OLK and OSCC increased in turn and inkeep with the results of microarray.Conclusion:Abnormal expression of angiogenic genes is closely related to oral carcinogenesis.
作者
吴苏宁
杨溪
刘伟
WU Suning;YANG Xi;LIU Wei(Department of Stomatology,Jiangnan University Affiliated the Third People's Hospital,Jiangsu Province,Wuxi 214000,China;Department of Oral and Maxillofacial-Head and Neck Oncology,Shanghai Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,National Clinical Research Center for Oral Diseases,Shanghai Key Laboratory of Stomatology&Shanghai Research Institute of Stomatology)
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2021年第3期337-341,共5页
Journal of Practical Stomatology
基金
国家自然科学基金(编号:82074502)。
关键词
口腔白斑
鳞癌
血管生成
VEGFA
异常增生
Oral leukoplakia
Squamous cell carcinoma
Angiogenesis
VEGFA
Dysplasia
