云南热区香茅草总黄酮及矿质元素含量

Evaluation of total flavonoids and mineral elements in Cymbopogon citratus in the Yunnan tropical area

以云南热区10个不同产地的香茅草(Cymbopogon citratus)为试材,利用正交设计优化香茅草中总黄酮的提取工艺条件,研究不同产地香茅草中总黄酮与矿质元素含量及其相关性.结果显示,总黄酮的最佳提取工艺条件为65%乙醇、75℃、1:35(g/mL)料液比、提取4 h,得率达到最高值.产地德宏的香茅草中总黄酮的含量最高,为7.458%,产地南华的含量最低,为3.605%;不同产地香茅草13种矿质元素中,含量差异明显,其中K、Ca、Mg、P和S含量最为丰富,为1058.33mg/kg以上,产地德宏的香茅草中K元素含量高达29647.5mg/kg,而Cr元素的含量仅为5.83mg/kg,且总黄酮含量最高的产地德宏的香茅草中矿质元素K的含量也最高.相关性研究发现香茅草中总黄酮含量与Ca呈负相关,相关系数为-0.500,与K呈正相关,相关系数为0.480.系统聚类分析发现产地保山、双江、红河、德宏、南华中矿质元素含量与总黄酮含量之间具有相关性,说明这些产地香茅草种质中矿质元素、总黄酮含量具有相似性.本研究表明云南热区香茅草种质中黄酮、不同矿质元素含量具有差异,总黄酮与矿质元素含量之间存在一定的相关性,矿质元素可能影响总黄酮的形成和积累;结果可为云南热区香茅草资源的种质筛选、良种培育及产品开发提供科学依据.(图2表5参32)

Using citronella,Cymbopogon citratus,from ten different habitats in the Yunnan tropical area as test materials,the extraction conditions for total flavonoids from C.citratus were optimized by orthogonal design,and the content and correlation analysis of total flavonoids and mineral elements in C.citratus from the different habitats were studied.The results showed that the optimum extraction conditions for total flavonoids were as follows:65%ethanol,75°C,1∶35(g/mL)solid-liquid ratio,and 4 h,where the yield reached the highest value.The total flavonoid content in citronella from Dehong was the highest at 7.458%,and was the lowest in Nanhua at 3.605%.There were obvious differences in the content of 13 mineral elements in citronella from different habitats,among which K,Ca,Mg,P,and S were the most abundant at over 1058.33 mg/kg.The content of K in citronella from Dehong was as high as 29647.5 mg/kg,while the content of Cr was only 5.83.The content of mineral element K in Dehong,which had the highest total flavonoid content,was also the highest.A correlation study found that the total flavonoid content in Cymbopogon citratus was negatively correlated with Ca,with a correlation coefficient of-0.500,and positively correlated with K,with a correlation coefficient of 0.480.Cluster analysis showed a correlation between mineral element content and total flavonoid content in plants from the Baoshan,Shuangjiang,Honghe,Dehong,and Nanhua regions,which indicated that the mineral elements and total flavonoids in citronella germplasms from these habitats were similar.The results showed that the contents of flavonoids and different mineral elements in citronella germplasms in the Yunnan tropical area differed,and there was a certain correlation between the contents of total flavonoids and mineral elements,which might affect the formation and accumulation of flavonoids.This study provides a scientific basis for germplasm screening,improved seed cultivation,and product development of citronella resources in the Yunnan tropical area.