以离子液体为流动相添加剂的HPLC法测定30批不同产地吴茱萸中8种生物碱的含量

Determination of 8 alkaloids in 30 batches of Euodiae fructus from different producing areas by HPLC with ionic liquid as mobile phase additive

目的建立吴茱萸药材中去氢吴茱萸碱、二氢吴茱萸次碱、吴茱萸碱、吴茱萸次碱、1-甲基-2-[(Z)-5-十一碳烯]-4-(1H)-喹诺酮、吴茱萸卡品碱、1-甲基-2-[(6Z,9Z)-6,9-十五碳二烯]-4-(1H)-喹诺酮、二氢吴茱萸卡品碱8种生物碱的定量分析方法。方法采用色谱柱为Waters symmetry C18(250 mm×4.6 mm, 5μm)柱,流动相为乙腈-体积分数0.1%磷酸水溶液(含3 mmol·L-1的1-丁基-3-甲基咪唑氯化盐离子液体),梯度洗脱,流速为1.0 mL·min-1,紫外检测波长为254 nm,柱温30℃。结果 8种生物碱分别在质量浓度4.256~212.8 mg·L-1、2.988~149.4 mg·L-1、5.996~299.8 mg·L-1、4.092~204.6 mg·L-1、1.014~50.72 mg·L-1、2.030~101.5 mg·L-1、0.829 6~41.48 mg·L-1、1.982~99.10 mg·L-1内线性关系良好,方法学考察结果符合要求。结论本方法为提升吴茱萸的质量标准提供实验依据。

Objective To establish a quantitative analysis method for 11 index components of dehydroevodipine, dihydroevodipine, evodia, evodipine, 1-methyl-2-[(Z)-5-undecarbonene]-4-(1H)-quinolone, evocarpine, 1-methyl-2-[(6Z,9Z)-6,9-decarboxylene]-4-(1H)-quinolone, dihydroevocarpine in Euodiae fructus.Methods The Waters symmetry C18(250 mm × 4.6 mm, 5 μm) column was used for the separation by acetonitrile-0.1% phosphoric acid solution(containing 3 mmol·L-1 of C4 minCl ionic liquid) with gradient elution.The flow rate was 1.0 mL·min-1.UV detection wavelength was 254 nm.And the column temperature was at 30 ℃.Results The linear relationship of eight alkaloids were within the range of 4.256-212.8 mg·L-1,2.988-149.4 mg·L-1,5.996-299.8 mg·L-1,4.092-204.6 mg·L-1,1.014-50.72 mg·L-1,2.030-101.5 mg·L-1,0.8296-41.48 mg·L-1 and 1.982-99.10 mg·L-1.The results of the methodological investigation met the requirements.Conclusion This study establishes a simple, rapid and accurate method for simultaneous determination of 8 alkaloids in Euodiae fructus, which can provide the basis for improving the quality standard of evodia officinalis.