重组酶聚合酶扩增技术研究进展

Advances in the research of recombinase polymerase amplification technology

新冠疫情的暴发使核酸检测家喻户晓,荧光定量聚合酶链反应(PCR)技术是此次防疫使用最多的核酸检测方法,但因对操作人员、仪器及场地的要求较高限制了其在某些资源匮乏或者实验室外场景的应用。恒温扩增技术特别是重组酶聚合酶扩增(RPA)技术具有反应条件温和、灵敏度高、特异性好、反应时间短等优点,在多种病原微生物的快速检测中具有较好的应用前景。该文对RPA技术的发展及应用进行回顾与总结,以期为该技术的深入研究及推广提供参考。

The outbreak of coronavirus disease 2019 has made nucleic acid detection widely known.Fluorescent quantitative polymerase chain reaction(PCR)technique is the most commonly used nucleic acid detection method in this epidemic control.However,its requirements on operators,instruments and sites limited its application in some scenarios with lower resources or outside laboratories.Isothermal amplification technology,especially recombinase polymerase amplification(RPA)technology,has the advantages of mild reaction conditions,high sensitivity,excellent specificity and short reaction time,which let it has a good application prospect in the rapid detection of a variety of pathogenic microorganisms.In this paper,the development and application of RPA technology are reviewed and summarized to provide a reference for the further research and promotion of this technology.