蛇床子素对骨肉瘤细胞增殖、迁移和侵袭的抑制作用及其机制

The Inhibitory Efficacy of Osthole on the Proliferation,Migration and Invasion of Osteosarcoma Cells and Its Mechanisms

目的:探讨蛇床子素(Osthole,OST)对人骨肉瘤细胞U2-OS增殖、迁移、侵袭的抑制作用及其机制。方法:培养人骨肉瘤细胞U2-OS,采用不同浓度的OST处理细胞,四甲基偶氮唑盐(MTT)法检测细胞增殖情况,克隆形成实验检测细胞克隆形成的能力,细胞划痕实验观察细胞迁移能力,Transwell小室法检测细胞侵袭作用,Western-Blot法检测PTEN、pAKT、AKT蛋白的表达。结果:MTT法结果表明OST能够抑制人骨肉瘤细胞U2-OS的增殖并呈剂量依赖性;克隆形成实验结果表明OST能够抑制U2-OS细胞的克隆形成能力;细胞划痕实验和细胞侵袭实验结果显示OST处理后细胞的迁移和侵袭能力明显降低;Western-Blot结果显示OST处理后PTEN蛋白的表达明显增加,pAKT蛋白的表达明显下降,并呈现一定的剂量依赖性。结论:OST可抑制人骨肉瘤细胞U2-OS增殖、迁移、侵袭,其可能的作用机制与调控PTEN-AKT信号通路有关。

Objective:To explore the inhibitory efficacy of osthole(OST)on the proliferation,migration and invasion of human osteosarcoma U-2 OS cells and its mechanisms.Methods:U2-OS were cultured and treated with the different doses of OST.The efficacy of OST on cell proliferation was detected by MTT assay.The efficacy of OST on cell colony formation was detected by colony formation assay.The efficacy of OST on cell migration was tested by wound healing assay.The efficacy of OST on cell invasion was detected by Transwell invasion assay.The efficacy of OST on the expression of PTEN,pAKT and AKT proteins was assessed by Western-Blot assay.Results:MTT assay showed that OST could inhibit the proliferation of U2-OS cells in a dose-dependent manner.The colony formation assay showed that OST could significantly inhibit the colony formation capacity of U2-OS cells.The wound healing and Transwell invasion assays showed that OST could greatly decreased the cell migration and invasion of osteosarcoma cells.The Western-Blot assay showed that OST could significantly increase the expression of PTEN,and decreased the expression of pAKT protein in a dose-dependent manner.Conclusion:OST could inhibit the proliferation,migration and invasion of human osteosarcoma U2-OS cells,which may be associated with the regulation of PTEN-AKT signaling.