电针对帕金森病小鼠结肠及黑质α-syn表达的影响

Effect of electroacupuncture on expression of α-syn in colon and substantia nigra of Parkinson’s disease mice

目的:观察电针干预对帕金森病(PD)模型小鼠行为学、结肠及中脑黑质中α-突触核蛋白(α-syn)表达的影响,探讨早期电针干预PD的效应及其机制。方法:C57BL/6J小鼠随机分为空白组、模型组、电针组,每组8只。除空白组外,其余各组均皮下注射鱼藤酮构建PD小鼠模型。电针组于造模当天开始针刺或电针干预"神庭""天枢""曲池""上巨虚",频率2 Hz,电流强度1 mA,10 min/次,1次/d, 7 d为1个疗程,共干预8个疗程。分别在造模前、造模中和造模结束后对各组小鼠进行悬挂、爬杆测试。治疗结束后采用Western blot、免疫组织化学法、免疫荧光法检测各组小鼠结肠和中脑黑质中α-syn表达的变化。结果:造模8周后,模型组小鼠精神萎靡,毛色暗淡,出现怕冷、蜷缩等症状。与空白组比较,造模6周后模型组小鼠悬挂评分明显降低(P<0.01),爬杆时间显著延长(P<0.01),结肠和黑质中α-syn蛋白表达量显著升高(P<0.01)。与模型组比较,电针组小鼠精神状态良好,皮毛光泽,悬挂评分升高(P<0.01),爬杆时间缩短(P<0.05),结肠和黑质中α-syn的表达明显下降(P<0.01)。结论:电针早期干预可延缓鱼藤酮诱导的PD模型小鼠行为障碍发生的时间,其机制可能与降低结肠及中脑黑质中α-syn的表达有关。

Objective To observe the effect of electroacupuncture(EA) intervention on the behavior, colon and midbrain substantia nigra α-synuclein(α-syn) expression in Parkinson’s disease(PD) mice, so as to explore the mechanism of early EA on prevention of PD. Methods C57 BL/6 J mice were randomly divided into blank, model and EA groups, with 8 mice in each group. The PD mouse model was established by subcutaneous injection of rotenone. Mice in the EA group received EA(2 Hz, 1 mA) at "Quchi"(LI11), "Shangjuxu"(ST37) and acupuncture at "Shenting"(GV24)and "Tianshu"(ST25) for 10 min once daily, with 7 d for a course, a total of 8 courses. Behavioral tests of hanging and climbing poles were carried out before, during and after modeling. The expressions of a-syn in substantia nigra and colon were detected by Western blot and immunohistochemistry or immunofluorescence. Results After 8 weeks of modeling, the mice in the model group showed symptoms such as listlessness, dim hair color, coldness and curling up. Compared with the blank group, the hanging score of the model group was significantly lower(P<0.01), the pole climbing time and the expressions of α-syn protein in colon and substantia nigra were significantly increased(P<0.01). Following intervention, mice in the EA group were always in good mental state, with shiny fur and stable body weight. The hanging scores was significantly higher and the pole climbing time and the expressions of α-syn protein in colon and substantia nigra were significantly down-regulated(P<0.05, P<0.01) in the EA group relevant to the model group. Conclusion Early EA intervention can delay the onset of PD induced by rotenone, which may be related to its effect in reducing the expression of α-syn in midbrain substantia nigra and colon.