异源表达小麦TaSAP7-B基因增强拟南芥对盐和渗透胁迫的敏感性

Expression of Wheat TaSAP7-B Gene in Arabidopsis Enhances Susceptibility to Salt and Osmotic Stresses

胁迫相关蛋白(stress associated protein,SAP)是指由A20结构域和(或)AN1结构域组成的一类锌指蛋白,参与调控植物对不同非生物胁迫的应答。本研究从小麦(Triticum aestivum L.)的B基因组中分离得到一个胁迫相关蛋白基因TaSAP7-B,该基因全长543 bp,编码180个氨基酸,预测蛋白分子量为19.66 kD,等电点为8.33。为进一步研究TaSAP7-B基因的生物学功能,将TaSAP7-B基因构建到植物表达载体pCAMBIA1300上并进行拟南芥的转化,相对于野生型对照,过表达TaSAP7-B基因拟南芥对盐胁迫和渗透胁迫更加敏感。上述结果表明,TaSAP7-B基因是盐胁迫和渗透胁迫反应的负调控因子。

Stress associated protein(SAP)is the A20/AN1 zinc-finger protein which involves in regulation the response to various abiotic stresses in plants.In this study,a stress associated protein gene,TaSAP7-B,was isolated from wheat(Triticum aestivum L.)genome B.The full-length sequence of TaSAP7-B gene was 543 bp,encoding a protein of 180 amino acids with a relative molecular weight of 19.66 kD and an isoelectric point of 8.33.To validate TaSAP7-B gene function,it was constructed into the plant expression vector pCAMBIA1300 and transformed into Arabidopsis thaliana.Compared to the wild type,overexpression of TaSAP7-B in Arabidopsis significantly enhanced susceptibility to salt and osmotic stresses.The above findings indicated that TaSAP7-B may function as a negative factor in plant response to salt and osmotic stresses.