杜鹃兰丛生芽诱导发生的适宜条件

Appropriate Condition for Cluster Buds Induction of Cremastra appendiculata

为筛选杜鹃兰丛生芽诱导发生的适宜条件,以杜鹃兰种子无菌萌发的原球茎为外植体材料,探究了不同基础培养基、植物生长调节剂以及抗褐化剂的种类及其浓度等因素对杜鹃兰丛生芽诱导的影响。结果表明:1/4MS培养基为适宜丛生芽发生的基本培养基;植物生长调节剂噻苯隆(TDZ)浓度为2.0 mg/L、吲哚乙酸(IAA)浓度为0.2 mg/L时,丛生芽诱导率较高,分别为72.58%和55.63%;抗褐化剂为聚乙烯吡咯烷酮(PVP),浓度为2000 mg/L时,丛生芽诱导率较高,为79.90%。杜鹃兰丛生芽诱导适宜条件为:1/4MS+TDZ 2.0 mg/L+IAA 0.1 mg/L+PVP 2000 mg/L,诱导率可达81.16%。为建立杜鹃兰快速繁殖技术体系、实现其规模化人工种植提供依据。

To screen for suitable conditions for cluster bud induction of Cremastra appendiculata.The aseptic germination protocorms of C.appendiculata seeds were used as explant materials to explore the effects of different basic media,types of plant growth regulators,anti-browning agents and their concentrations on the induction of cluster buds of C.appendiculata.The results showed that:1/4 MS medium was the suitable basic medium for cluster buds growing;when the concentration of plant growth regulator TDZ was 2.0 mg/L and the concentration of IAA was 0.2 mg/L,the induction rates of cluster bud were higher which are 72.58%and 55.63%respectively;when the anti-browning agent was PVP and its concentration is 2000 mg/L,the induction rate of cluster buds was higher which is 79.90%.The suitable conditions for C.appendiculata cluster buds induction are:1/4 MS+TDZ 2.0 mg/L+IAA 0.1 mg/L+PVP 2000 mg/L,and the induction rate can reach 81.16%.To provide a basis for establishing a rapid propagation technology system of C.appendiculata and realizing its large-scale artificial cultivation.