‘六月早’蜜柚叶绿体基因组及其特征分析

Chloroplast Genome Sequence and Characteristics Analysis of Citrusmaxima ‘Liuyuezao’

‘六月早’蜜柚(Citrus maxima‘Liuyuezao’)为国内重要的柚品种琯溪蜜柚早熟芽变品种。以‘六月早’蜜柚为材料,利用二代测序进行全基因组重测序,从中组装获取叶绿体基因组并对其进行注释。结果表明:组装获得的‘六月早’蜜柚的叶绿体基因组全长160186 bp,四分体结构由大单拷贝区(large single copy,LSC)、小单拷贝区(small single copy,SSC)和反向重复区(inverted repeat,IR)组成,3个分区的长度分别为87939、18395、26926 bp。注释得到133个基因,其中包含89个编码基因,37个tRNA和8个rRNA。共识别到31个短串联重复序列,101个SSR位点。将已公开发表的29个芸香科叶绿体基因组使用最大似然法进行系统发育关系的构建,结果表明,‘六月早’蜜柚与甜橙(C.sinensis)、柠檬(C.limon)和C.platymamma的亲缘关系较近。

Citrus maxima‘Liuyuezao’is a precocious bud mutation of Guanximiyou,an important pomelo variety of China.In this study,the whole genome resequencing of C.maxima‘Liuyuezao’was conducted using next-generation sequencing.The chloroplast(cp)genome was assembled and annotated.The cp genome had a total length of 160186 bp,the tetrad structed was consisted with large single copy(LSC),small single copy(SSC)and inverted repeat(IR),with the length in 87939 bp,18395 bp and 26926 bp respectively.The cp genome contained a total of 133 genes,including 89 protein-coding genes,37 tRNA genes and 8 tRNA genes.We identified 31 tandem repeat and 101 SSRs.Combined with 29 published cp genome sequences of Rutaceae,we used the maximum likelihood method to reconstruct the phylogenetic relationship of Rutaceae.The result showed that C.maxima‘Liuyuezao’had a close relationship with C.sinensis,C.limon and C.platymamma.