8个玉米ZmDOFs基因对非生物胁迫响应的表达分析

Expression Profiling of Eight ZmDOFs Genes Responding to Abiotic Stresses in Maize

转录因子家族是由含有锌指结构的DOF结构域组成,构成植物特有的转录因子家族之一,在植物的生长发育进程中诸如信号转导、形态建成、抵御环境胁迫等方面都发挥着重要的作用。本研究利用定量PCR技术,探测了8个ZmDOFs基因在玉米6个不同组织的表达模式,结果表明,8个ZmDOFs基因具有不同的组织特异性表达模式,4个主要在幼穗中表达,2个主要在雄花中表达,ZmDOF28主要在苞叶中表达,而ZmDOF38在多种组织中表达,表明在玉米进化的进程中8个ZmDOFs基因表达模式的保守性和特异性。盐胁迫和干旱胁迫时,分别有7个和6个ZmDOFs基因受到明显的诱导,表明ZmDOFs基因广泛参与玉米应答盐胁迫或干旱胁迫响应途径。在铵态氮胁迫和硝态氮胁迫时,分别有7个和8个ZmDOFs基因的表达受到了明显的改变,表明ZmDOFs基因参与玉米应答氮胁迫响应途径,体现了在其历史进化进程中的表达模式和功能的保守性与独特性。本研究为后续研究ZmDOFs基因的功能提供了参考。

The DNA-binding one finger(DOF)transcription factor family is a major family of plant-specific transcription factors containing the DOF domain.These transcription factors are involved in a variety of functions of importance for different biological processes in plants.In the current study,an overview of 8 DOFs genes in maize is presented,including the gene structure,chromosome location,phylogeny,proten motif and expression pattern in responses to various abiotic stresses.Expression patterns indicated that the eight ZmDOFs had tissue specific expression patterns,indicating these genes are crucial in maize growth and development.Moreover,we examined the transcriptional levels of the eight ZmDOFs under salt and PEG6000 stress treatments,seven ZmDOFs and six ZmDOFs were up-regulated after salt and PEG6000 treatment,respectively.We also analyzed the eight ZmDOFs profilling under ammonium and nitrate stress treatment,seven and eight ZmDOFs significantly responded to these two stresses,indicating they are associated with nitrogen stresses responses.The results would provide a very useful reference for the cloning and functional analysis of the members of this gene family in maize and other species.